Redondo Fernando, Hurtado-Marcos Carolina, Izquierdo Fernando, Cuéllar Carmen, Fenoy Soledad, Sáez Yanira, Magnet Ángela, Galindo-Regal Lorena, Uribe Natalia, López-Bañeres Manuel, Jiménez Ana Isabel, Llombart-Cussac Antonio, Del Águila Carmen, Andreu-Ballester Juan Carlos
Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, 28660 Boadilla del Monte, Spain.
Department of Microbiology and Parasitology, Complutense University, 28040 Madrid, Spain.
Cancers (Basel). 2022 Oct 29;14(21):5342. doi: 10.3390/cancers14215342.
Microsporidia are opportunistic intracellular parasites, generating serious pathology in individuals with a compromised immune system. Infection by microsporidia inhibits p53 and Caspase 3, proteins involved in apoptosis and the cell cycle, which are vital in the malignant process of epithelial cells. The presence of microsporidia in the intestinal tissues of 87 colon cancer (CC) patients and 25 healthy controls was analyzed by real-time PCR and an immunofluorescence antibody test. Anti-Encephalitozoon antibodies were analyzed in serum samples by ELISA (enzyme linked immunosorbent assay). In 36 (41.3%) CC cases, microsporidia infections were identified in their tissues vs. no cases among control subjects (p < 0.0001). An increase in IgG and IgE anti-Encephalitozoon antibodies was found in patients with CC, which would demonstrate continuous and previous contact with the parasite. The high prevalence of microsporidia in tissues and the seroprevalence in patients with CC suggest a relationship between microsporidia and the etiopathogenesis of CC.
微孢子虫是机会性细胞内寄生虫，会在免疫系统受损的个体中引发严重病变。微孢子虫感染会抑制参与细胞凋亡和细胞周期的蛋白质p53和半胱天冬酶3，而这些蛋白质在上皮细胞的恶性病变过程中至关重要。通过实时聚合酶链反应和免疫荧光抗体检测分析了87例结肠癌（CC）患者和25名健康对照者肠道组织中微孢子虫的存在情况。通过酶联免疫吸附测定（ELISA）分析血清样本中的抗脑胞内原虫抗体。在36例（41.3%）CC病例的组织中鉴定出微孢子虫感染，而对照受试者中未发现病例（p < 0.0001）。在CC患者中发现抗脑胞内原虫抗体IgG和IgE增加，这表明与该寄生虫存在持续和既往接触。CC患者组织中微孢子虫的高感染率和血清阳性率表明微孢子虫与CC的病因发病机制之间存在关联。
