Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
Exp Cell Res. 2021 Jan 15;398(2):112441. doi: 10.1016/j.yexcr.2020.112441. Epub 2020 Dec 16.
The Yangtze River Delta white goat is a sole goat species that can naturally produce superior-quality brush hair. It's worth to mention that study the developmental mechanism of goat hair follicle stem cells is vital for future breed preservation and molecular breeding. In this study, we successfully isolated hair follicle stem cells from the skin tissue of fetal sheep neck spine, and harvested superior-quality and normal-quality brush hair goat tissue. The expression of miR-31-5p in goat hair follicle stem cells was verified by qPCR and Western blot. The effects of overexpression or inhibition of miR-31-5p on the proliferation and apoptosis of hair follicle stem cells were detected by EdU, CCK-8, flow cytometry, etc. miR-31-5p can significantly improve cell proliferation and inhibit cell apoptosis by targeting RASA1 and upregulating MAP3K1 level, whereas miR-31-5p knockdown led to an opposite effect. These results reveal a miR-31-5p-associated regulatory network between miR-31-5p and RASA1/MAP3K1 during the progression of superiorquality brush hair traits.
长江三角洲白山羊是一种能天然生产优质笔毛的特有山羊品种。值得一提的是，研究山羊毛囊干细胞的发育机制对于未来的品种保护和分子育种至关重要。在这项研究中，我们成功地从胎儿羊颈脊柱的皮肤组织中分离出毛囊干细胞，并采集了优质和正常笔毛山羊组织。通过 qPCR 和 Western blot 验证了 miR-31-5p 在山羊毛囊干细胞中的表达。通过 EdU、CCK-8、流式细胞术等检测 miR-31-5p 过表达或抑制对毛囊干细胞增殖和凋亡的影响。miR-31-5p 可以通过靶向 RASA1 并上调 MAP3K1 水平来显著促进细胞增殖并抑制细胞凋亡，而 miR-31-5p 敲低则产生相反的效果。这些结果揭示了在优质笔毛性状进展过程中 miR-31-5p 与 RASA1/MAP3K1 之间的 miR-31-5p 相关调控网络。
