College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing 102206, China.
Genes (Basel). 2022 Oct 30;13(11):1979. doi: 10.3390/genes13111979.
Houtt., a traditional medicine herb of the Polygonaceae family, has been used since ancient times in China due to its various pharmacological effects. Chloroplast genomes are conservative and play an essential role in population diversity analysis. However, there are few studies on the chloroplast genome of . In this study, the complete chloroplast genomes of three from different regions were performed by next-generation sequencing technology. The results revealed that the lengths of the three chloroplast genomes are between 163,371163,372 bp, and they have a highly conserved structure with a pair of inverted repeat (IR) regions (31,121 bp), a large single-copy (LSC) region (87,57187,572 bp), and a small single-copy (SSC) region (13,558 bp). In total, 132 genes were annotated, including 8 rRNA genes, 37 tRNA genes, and 87 protein-coding genes. The phylogenetic analysis strongly revealed that 13 populations of form a monophyly, and (Polygonaceae) is its closest species. The two species diverged at ~20.47 million years ago, and in China could be further divided into two major groups based on genetic structure analysis. In addition, several potential loci with suitable polymorphism were identified as molecular markers. Our study provides important genetic resources for further development and utilization of germplasm, as well as some new insights into the evolutionary characteristics of this medicinal plant.
Houtt.，一种来自蓼科的传统药用植物，由于其多种药理学作用，自古以来就在中国被使用。叶绿体基因组保守，在种群多样性分析中起着重要作用。然而，关于 的叶绿体基因组研究较少。本研究通过下一代测序技术对来自不同地区的三种 进行了完整的叶绿体基因组测序。结果表明，三种叶绿体基因组的长度在 163,371163,372 bp 之间，它们具有高度保守的结构，一对反向重复（IR）区（31,121 bp）、一个大单拷贝（LSC）区（87,57187,572 bp）和一个小单拷贝（SSC）区（13,558 bp）。总共注释了 132 个基因，包括 8 个 rRNA 基因、37 个 tRNA 基因和 87 个蛋白质编码基因。系统发育分析强烈表明，13 个 种群形成一个单系群，而 （Polygonaceae）是其最接近的物种。这两个物种在大约 2047 万年前分化，基于遗传结构分析，中国的 可以进一步分为两个主要群体。此外，还鉴定了一些具有合适多态性的潜在基因座作为分子标记。我们的研究为进一步开发和利用 的种质资源提供了重要的遗传资源，并为这种药用植物的进化特征提供了一些新的见解。
