Wang Cui, Su Kaidi, Li Qiwen, Sun Rui, Liu Haoyu, Du Jingxuan, Li Jinping, Liu Likuan
Qinghai Provincial Key Laboratory of Medicinal Plant and Animal Resources of Qinghai-Tibet Plateau, School of Life Sciences, Qinghai Normal University, Xining 810008, China.
Academy of Plateau Science Sustainability, Xining 810008, China.
Genes (Basel). 2025 Jun 30;16(7):789. doi: 10.3390/genes16070789.
var. (Saxifragaceae) is recognized as a genuine medicinal material from the Qinghai-Tibet Plateau in China. This paper presents the chloroplast (cp) genome of var. , marking the first report for this genus. The Tibetan medicinal plants documented in 'Chinese Medicinal Plant Resources' are associated with their chloroplast genomes and medicinal mechanisms.
In order to resolve any potential ambiguity in conventional classifications, this study reconstructs the evolutionary position of var. within the genus by comparing its chloroplast genetic information with that of other groupings.
The chloroplast genome of var. was sequenced using the Illumina NovaSeq 6000 platform. Subsequent sequence assembly, annotation, and characterization were performed using bioinformatics analysis. The NJ phylogenetic tree was constructed using MEGA 7.0 software.
The complete chloroplast genome of var. is 146,549 bp in length, comprising four subregions: a large single-copy (LSC) region of 79,318 bp and a small single-copy (SSC) region of 16,390 bp, separated by a pair of inverted repeat (IR) regions each 25,421 bp long. This cp genome contains 131 genes, including 86 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The overall GC content is 38.1%. Phylogenetic analysis based on 20 cp genomes indicates that var. is closely related to and .
某某变种（虎耳草科）被认为是中国青藏高原的一种地道药材。本文展示了某某变种的叶绿体（cp）基因组，这是该属的首次报道。《中国药用植物资源》中记载的藏药植物与其叶绿体基因组和药用机制相关。
为了解决传统分类中可能存在的模糊性，本研究通过将某某变种的叶绿体遗传信息与其他类群的信息进行比较，重建其在该属中的进化位置。
使用Illumina NovaSeq 6000平台对某某变种的叶绿体基因组进行测序。随后使用生物信息学分析进行序列组装、注释和特征描述。使用MEGA 7.0软件构建NJ系统发育树。
某某变种的完整叶绿体基因组长度为146,549 bp，包括四个亚区域：一个79,318 bp的大单拷贝（LSC）区域和一个16,390 bp的小单拷贝（SSC）区域，由一对长度均为25,421 bp的反向重复（IR）区域隔开。该cp基因组包含131个基因，包括86个蛋白质编码基因、37个tRNA基因和8个rRNA基因。总体GC含量为38.1%。基于20个cp基因组的系统发育分析表明，某某变种与某某和某某密切相关。
