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药用植物(蓼科)的完整叶绿体基因组及其在蓼亚科内的系统发育意义。

The complete chloroplast genome of the medicinal plant (Polygonaceae) and its phylogenetic implications within the subfamily Polygonoideae.

作者信息

Ye Xiaofeng, Lin Jiuguo, Zhou Manjia, He Xiangyu, Yan Meixiu, Cheng Rubin

机构信息

College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, China.

出版信息

Mitochondrial DNA B Resour. 2021 Apr 26;6(4):1563-1565. doi: 10.1080/23802359.2021.1917313.

Abstract

Siebold & Zucc. is a well-known and widely used medical plant to treat arthritis, gout and inflammation. In this study, we determined the complete chloroplast genome sequence of from Zhejiang Province. The assembled chloroplast (cp) genome was 163,183 bp in length, containing two inverted repeated (IR) regions of 30,859 bp each, a large single copy (LSC) region of 87,905 bp, and a small single copy (SSC) region of 13,560 bp. The genome encodes 131 genes, consisting of 86 protein-coding, 37 tRNA, and eight rRNA genes. The overall GC content of is 37.53%, with the highest GC content of 41.27% in the IR region. The 86 protein-coding genes encode 27,597 amino acids in total, most of which use the initiation codon ATG, except the gene which starts with ACG. The length of the tRNA genes range from 48 bp to 88 bp, with the highest GC content of 62.16% in tRNA-Arg (ACG) and tRNA-Asp (GUC). A total of 66 simple sequence repeats are identified in the cp of Phylogenetic analysis indicated a sister relationship between and , suggesting a close genetic relationship between the genera of and . This work provides basic genetic resources for investigating the evolutionary status and population genetics of this important medicinal species.

摘要

Siebold & Zucc.是一种治疗关节炎、痛风和炎症的著名且广泛使用的药用植物。在本研究中,我们测定了来自浙江省的Siebold & Zucc.的完整叶绿体基因组序列。组装后的叶绿体(cp)基因组长度为163,183 bp,包含两个各为30,859 bp的反向重复(IR)区域、一个87,905 bp的大单拷贝(LSC)区域和一个13,560 bp的小单拷贝(SSC)区域。该基因组编码131个基因,包括86个蛋白质编码基因、37个tRNA基因和8个rRNA基因。Siebold & Zucc.的总体GC含量为37.53%,IR区域的GC含量最高,为41.27%。86个蛋白质编码基因共编码27,597个氨基酸,其中大多数使用起始密码子ATG,但 基因以ACG起始。tRNA基因的长度范围为48 bp至88 bp,tRNA-Arg(ACG)和tRNA-Asp(GUC)的GC含量最高,为62.16%。在Siebold & Zucc.的cp中总共鉴定出66个简单序列重复。系统发育分析表明Siebold & Zucc.与 之间存在姐妹关系,表明Siebold & Zucc.属与 属之间存在密切的遗传关系。这项工作为研究这种重要药用物种的进化地位和群体遗传学提供了基础遗传资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6afb/8078928/d803a46a1d8f/TMDN_A_1917313_F0001_B.jpg

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