Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.
Microb Cell Fact. 2022 Aug 2;21(1):152. doi: 10.1186/s12934-022-01865-7.
Scopoletin is a typical example of coumarins, which can be produced in plants. Scopoletin acts as a precursor for pharmaceutical and health care products, and also possesses promising biological properties, including antibacterial, anti-tubercular, anti-hypertensive, anti-inflammatory, anti-diabetic, and anti-hyperuricemic activity. Despite the potential benefits, the production of scopoletin using traditional extraction processes from plants is unsatisfactory. In recent years, synthetic biology has developed rapidly and enabled the effective construction of microbial cell factories for production of high value-added chemicals. Herein, this review summarizes the progress of scopoletin biosynthesis in artificial microbial cell factories. The two main pathways of scopoletin biosynthesis are summarized firstly. Then, synthetic microbial cell factories are reviewed as an attractive improvement strategy for biosynthesis. Emerging techniques in synthetic biology and metabolic engineering are introduced as innovative tools for the efficient synthesis of scopoletin. This review showcases the potential of biosynthesis of scopoletin in artificial microbial cell factories.
瑞香素是香豆素的典型代表，可以在植物中产生。瑞香素可用作药物和保健品的前体，具有多种有前景的生物活性，包括抗菌、抗结核、抗高血压、抗炎、抗糖尿病和降尿酸作用。尽管具有潜在的益处，但使用传统的植物提取工艺生产瑞香素并不理想。近年来，合成生物学发展迅速，使得有效构建微生物细胞工厂来生产高附加值化学品成为可能。本文综述了瑞香素在人工微生物细胞工厂中的生物合成进展。首先总结了瑞香素生物合成的两条主要途径。然后，综述了合成微生物细胞工厂作为生物合成的一种有吸引力的改进策略。介绍了合成生物学和代谢工程中的新兴技术，作为高效合成瑞香素的创新工具。本文综述展示了人工微生物细胞工厂中瑞香素生物合成的潜力。
