Penn State Health Milton S Hershey Medical Center, Department of Pediatrics, Hershey, PA 17033, USA.
Department of Pediatrics, Penn State Health Children's Hospital, 500 University Drive, Hershey, PA 17033, USA.
Genes (Basel). 2022 Nov 3;13(11):2024. doi: 10.3390/genes13112024.
Food reactions (FR) are multifactorial and impacted by medical, demographic, environmental, and immunologic factors. We hypothesized that multi-omic analyses of host-microbial factors in saliva would enhance our understanding of FR development. This longitudinal cohort study included 164 infants followed from birth through two years. The infants were identified as FR ( = 34) or non-FR ( = 130) using the Infant Feeding Practice II survey and medical record confirmation. Saliva was collected at six months for the multi-omic assessment of cytokines, mRNAs, microRNAs, and the microbiome/virome. The levels of one miRNA (miR-203b-3p, adj. = 0.043, V = 2913) and one viral phage (Proteus virus PM135, adj. = 0.027, V = 2955) were lower among infants that developed FRs. The levels of one bacterial phylum (Cyanobacteria, adj. = 0.048, V = 1515) were higher among infants that developed FR. Logistical regression models revealed that the addition of multi-omic features (miR-203b-3p, Cyanobacteria, and Proteus virus PM135) improved predictiveness for future FRs in infants ( = 0.005, X = 12.9), predicting FRs with 72% accuracy (AUC = 0.81, sensitivity = 72%, specificity = 72%). The multi-omic analysis of saliva may enhance the accurate identification of infants at risk of FRs and provide insights into the host/microbiome interactions that predispose certain infants to FRs.
食物反应（FR）是多因素的，受医学、人口统计学、环境和免疫学因素的影响。我们假设，对唾液中宿主-微生物因素的多组学分析将增强我们对 FR 发展的理解。这项纵向队列研究包括 164 名婴儿，从出生到两岁进行随访。通过使用婴儿喂养实践 II 调查和医疗记录确认，将婴儿确定为 FR（=34）或非 FR（=130）。在六个月时收集唾液，以进行细胞因子、mRNA、microRNA 和微生物组/病毒组的多组学评估。在发生 FR 的婴儿中，有一种 miRNA（miR-203b-3p，adj. =0.043，V=2913）和一种病毒噬菌体（Proteus 病毒 PM135，adj. =0.027，V=2955）的水平较低。在发生 FR 的婴儿中，有一种细菌门（蓝细菌，adj. =0.048，V=1515）的水平较高。逻辑回归模型显示，多组学特征（miR-203b-3p、蓝细菌和 Proteus 病毒 PM135）的加入提高了对未来 FR 婴儿的预测能力（=0.005，X=12.9），预测 FR 的准确率为 72%（AUC=0.81，敏感性=72%，特异性=72%）。唾液的多组学分析可能增强对 FR 风险婴儿的准确识别，并深入了解使某些婴儿易患 FR 的宿主/微生物组相互作用。
