School of Biological Sciences, University of Western Australia, Perth, WA 6009, Australia.
School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, China.
Genes (Basel). 2022 Nov 4;13(11):2037. doi: 10.3390/genes13112037.
Copy number variations (CNVs) are defined as deletions, duplications and insertions among individuals of a species. There is growing evidence that CNV is a major factor underlining various autoimmune disorders and diseases in humans; however, in plants, especially oilseed crops, the role of CNVs in disease resistance is not well studied. Here, we investigate the genome-wide diversity and genetic properties of CNVs in resistance gene analogues (RGAs) across eight lines. A total of 1137 CNV events (704 deletions and 433 duplications) were detected across 563 RGAs. The results show CNVs are more likely to occur across clustered RGAs compared to singletons. In addition, 112 RGAs were linked to a blackleg resistance QTL, of which 25 were affected by CNV. Overall, we show that the presence and abundance of CNVs differ between lines, suggesting that in , the distribution of CNVs depends on genetic background. Our findings advance the understanding of CNV as an important type of genomic structural variation in and provide a resource to support breeding of advanced canola lines.
拷贝数变异（CNVs）被定义为同种个体之间的缺失、重复和插入。越来越多的证据表明，CNV 是人类各种自身免疫性疾病和疾病的主要因素；然而，在植物中，特别是在油料作物中，CNV 在抗病性中的作用尚未得到很好的研究。在这里，我们研究了 8 个系中抗病基因类似物（RGAs）中 CNV 的全基因组多样性和遗传特性。在 563 个 RGAs 中总共检测到 1137 个 CNV 事件（704 个缺失和 433 个重复）。结果表明，与单体相比，CNV 更有可能在聚集的 RGAs 中发生。此外，112 个 RGAs 与黑胫病抗性 QTL 相关，其中 25 个受 CNV 影响。总体而言，我们表明 CNV 的存在和丰度在不同系之间存在差异，这表明在 中，CNV 的分布取决于遗传背景。我们的研究结果推进了对 CNV 作为 中一种重要基因组结构变异的理解，并为支持先进油菜品种的选育提供了资源。
