Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia.
Phytochemistry and Plant Systematics Department, National Research Centre, Giza 12622, Egypt.
Molecules. 2022 Oct 25;27(21):7242. doi: 10.3390/molecules27217242.
The liver is a crucial organ among body organs due to its wide functions, in particular, detoxification and metabolism. Exposure to detrimental chemicals or viral infections may provoke liver dysfunction and ultimately induce liver tissue damage. Finding natural substances for liver disease treatment to overcome the conventional treatments' side effects has attracted the attention of researchers worldwide. Our current work was conducted to investigate the hepato-therapeutic activities of essential oil (EO) isolated from flowers. EO was extracted using the hydro-distillation (HD) technique and its chemical composition was identified by GC/MS. Then, the hepatic treatment potential of extracted EO was evaluated in vivo against CCL in rats. HD of flowers yielded highly chemical constituents of EO along with significant antioxidant potential. A coherent molecular network was fashioned via the Global Natural Products Social Molecular Networking (GNPS) to visualize the essential components and revealed that the sesquiterpene ()--caryophyllene was the most predominant volatile constituent which accounted for 24.1%. The treatment of CCL led to significant induced oxidative stress markers malonaldehyde, total protein, and non-protein sulfhydryl, as well as elevated serum aminotransferase, gamma-glutamyl transferase, alkaline phosphatase, and bilirubin. In addition, it disrupted the level of lipid profile. The post-treatment using EO succeeded in relieving all toxic effects of CCl and recuperating the histopathological signs induced by CCL. Silymarin was used as a standard hepatoprotective agent. The obtained results demonstrated that the extracted EO exerted high protective activities against the toxicity of CCL. Moreover, the flowers EO can be used as a natural remedy to relieve many contemporary liver diseases related to oxidative stress.
肝脏是人体器官中至关重要的器官，因为它具有广泛的功能，特别是解毒和新陈代谢。有害化学物质或病毒感染的暴露可能会引起肝功能障碍，并最终导致肝组织损伤。寻找用于治疗肝病的天然物质以克服传统治疗方法的副作用引起了全球研究人员的关注。我们目前的工作旨在研究从花中提取的精油（EO）的肝治疗活性。使用水蒸馏（HD）技术提取精油，并通过 GC/MS 鉴定其化学成分。然后，在体内评估提取的 EO 对 CCL 在大鼠中的肝治疗潜力。花的 HD 产生了高度的 EO 化学成分，同时具有显著的抗氧化潜力。通过全球天然产物社会分子网络（GNPS）构建了一个连贯的分子网络，以可视化基本成分，并揭示了倍半萜烯（）-石竹烯是最主要的挥发性成分，占 24.1%。CCL 治疗导致显著诱导氧化应激标志物丙二醛、总蛋白和非蛋白巯基，以及血清转氨酶、γ-谷氨酰转移酶、碱性磷酸酶和胆红素升高。此外，它破坏了脂质谱的水平。用 EO 进行的后期治疗成功缓解了 CCl 的所有毒性作用，并恢复了 CCl 诱导的组织病理学迹象。水飞蓟素被用作标准的肝保护剂。所得结果表明，提取的 EO 对 CCL 的毒性具有很高的保护活性。此外，花的 EO 可作为一种天然药物，用于缓解许多与氧化应激有关的当代肝脏疾病。
