Programa de Pós-Graduação em Química, Universidade Federal do Pará, Belém 66075-900, Brazil.
Programa Institucional de Bolsas de Iniciação Científica, Universidade Federal do Pará, Belém 66075-900, Brazil.
Molecules. 2022 Oct 28;27(21):7337. doi: 10.3390/molecules27217337.
(Lauraceae), also known as "true cinnamon" or "Ceylon cinnamon" has been widely used in traditional folk medicine and cuisine for a long time. The systematics of presents some difficulties due to genetic variation and morphological similarity between other species. The present work aimed to find chemical and molecular markers of samples from the Amazon region of Brazil. The leaf EOs and the genetic material (DNA) were extracted from samples cultivated and commercial samples. The chemical composition of the essential oils from samples of cultivated (Cve1-Cve5) and commercial (Cve6-c-Cv9-c) was grouped by multivariate statistical analysis of Principal Component Analysis (PCA). The major compounds were rich in benzenoids and phenylpropanoids, such as eugenol (0.7-91.0%), benzyl benzoate (0.28-76.51%), ()-cinnamyl acetate (0.36-32.1%), and ()-cinnamaldehyde (1.0-19.73%). DNA barcodes were developed for phylogenetic analysis using the chloroplastic regions of the and genes, and - intergenic spacer. The - sequences provided greater diversity of nucleotides, and confirmed the identity of . The combination of DNA barcode and volatile profile was found to be an important tool for the discrimination of varieties and to examine the authenticity of industrial sources.
樟科（Lauraceae），也被称为“真肉桂”或“锡兰肉桂”，长期以来一直被广泛用于传统民间医学和烹饪中。由于遗传变异和其他樟属物种之间的形态相似性，对 的系统发育存在一些困难。本研究旨在寻找来自巴西亚马逊地区 的样本的化学和分子标记物。从栽培和商业样本中提取叶片精油和遗传物质（DNA）。通过主成分分析（PCA）的多元统计分析对栽培（Cve1-Cve5）和商业（Cve6-c-Cv9-c）样本的精油化学成分进行分组。主要化合物富含苯并呋喃和苯丙素类化合物，如丁香酚（0.7-91.0%）、苯甲酸苄酯（0.28-76.51%）、（）-肉桂酸乙酸酯（0.36-32.1%）和（）-肉桂醛（1.0-19.73%）。使用叶绿体基因和 - 内含子间隔区开发了用于系统发育分析的 DNA 条形码。- 序列提供了更多的核苷酸多样性，并且 - 证实了 的身份。DNA 条形码和挥发性图谱的结合被发现是区分 品种和检查工业来源真实性的重要工具。
