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从 中分离鉴定植物病原菌次生代谢产物。

Isolation and Identification of Secondary Metabolites Produced by Phytopathogenic Fungus from .

机构信息

State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China.

Hainan Key Laboratory of Tropical Microbe Resources, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences & Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute for Tropical Agricultural Resources, Haikou 571101, China.

出版信息

Molecules. 2022 Oct 29;27(21):7360. doi: 10.3390/molecules27217360.

Abstract

The secondary metabolites of the phytopathogenic fungus CC01 from were investigated. As a result, two new compounds, 5-acetyl-7-hydroxy-6- methoxybenzofuran-2(3)-one () and ()-2-(2,3-dihydrofuro [3,2-]pyridin-2-yl)propan-2-ol (), together with seven known compounds, 4,6,8-trihydroxy-3,4-dihydronaphthalen-1(2)-one (), 3,6,8-trihydroxy-3,4-dihydronaphthalen-1(2)-one (), curvulin acid (), 2-methyl-5-carboxymethyl- 7-hydroxychromone (), tyrosol (), -hydroxybenzoic acid () and cerevisterol (), were isolated from the fermentation extract by comprehensive silica gel, reverse phase silica gel, Sephadex-LH20 column chromatography and high-performance liquid chromatography (HPLC). The structures of these compounds were identified by using high-resolution electrospray mass spectrometry (HRESIMS), nuclear magnetic resonance spectroscopy (NMR), optical rotation, ultraviolet and infrared spectroscopy techniques and a comparison of NMR data with those reported in the literature. Compounds and were new compounds, and compounds - were discovered from this phytopathogenic fungus for the first time. Compounds - were tested for phytotoxicity against the fresh tender leaf of , and the results show that none of them were phytotoxic. Additionally, these compounds were subjected to an antimicrobial assay against three bacteria (, and ), but they showed no activity.

摘要

从 中分离得到的植物病原菌 CC01 的次生代谢产物。结果,分离得到了两个新化合物,5-乙酰基-7-羟基-6-甲氧基苯并呋喃-2(3)-酮 () 和 ()-2-(2,3-二氢呋喃并[3,2-]吡啶-2-基)丙-2-醇 (),以及 7 个已知化合物,4,6,8-三羟基-3,4-二氢萘-1(2)-酮 ()、3,6,8-三羟基-3,4-二氢萘-1(2)-酮 ()、曲古菌素酸 ()、2-甲基-5-羧甲基-7-羟基色酮 ()、酪醇 ()、-羟基苯甲酸 () 和麦角甾醇 ()。这些化合物的结构通过综合硅胶、反相硅胶、Sephadex-LH20 柱层析和高效液相色谱 (HPLC) 从发酵提取物中分离得到。通过高分辨电喷雾质谱 (HRESIMS)、核磁共振波谱 (NMR)、旋光、紫外和红外光谱技术以及与文献报道的 NMR 数据比较,鉴定了这些化合物的结构。化合物 和 是新化合物,化合物 - 首次从这种植物病原菌中发现。化合物 - 对 新鲜嫩叶进行了植物毒性测试,结果表明它们均无植物毒性。此外,这些化合物还进行了对三种细菌( 、 和 )的抗菌活性测定,但它们均无活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af6c/9657256/5d1f54267c3b/molecules-27-07360-g001.jpg

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