School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China.
Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Normal University, Wuhu 241002, China.
Molecules. 2022 Nov 2;27(21):7444. doi: 10.3390/molecules27217444.
Hollow mesoporous silica nanoparticles have been widely applied as a carrier material in the molecular imprinting process because of their excellent properties, with high specific surface area and well-defined active centers. However, these kinds of materials face the inevitable problem that they have low mass transfer efficiency and cannot be conveniently recycled. In order to solve this problem, this work has developed a composite hydrogel microsphere (MMHSG) encapsulated with hollow mesoporous imprinted nanoparticles for the selective extraction of 2'-deoxyadenosine (dA). Subsequently, the hollow mesoporous imprinted polymers using dA as template molecule and synthesized 5-(2-carbomethoxyvinyl)-2'-deoxyuridine (AcrU) as functional monomer were encapsulated in hydrogel. MMHSG displayed good performance in specifically recognizing and quickly separating dA, whereas no imprinting effect was observed among 2'-deoxyguanosine (dG), deoxycytidine (dC), or 5'-monophosphate disodium salt (AMP). Moreover, the adsorption of dA by MMHSG followed chemisorption and could reach adsorption equilibrium within 60 min; the saturation adsorption capacity was 20.22 μmol·g. The introduction of AcrU could improve selectivity through base complementary pairing to greatly increase the imprinting factor to 3.79. Therefore, this was a successful attempt to combine a hydrogel with hollow mesoporous silica nanoparticles and molecularly imprinted material.
中空介孔硅纳米粒子因其具有高比表面积和明确的活性中心等优异性能，已被广泛应用于分子印迹过程中作为载体材料。然而，这些材料面临着传质效率低且不便回收的固有问题。为了解决这个问题，本工作开发了一种复合水凝胶微球（MMHSG），其中封装了中空介孔印迹纳米粒子，用于选择性提取 2'-脱氧腺苷（dA）。随后，以 dA 为模板分子，5-(2-羧基乙烯基)-2'-脱氧尿苷（AcrU）为功能单体合成的中空介孔印迹聚合物被封装在水凝胶中。MMHSG 在特异性识别和快速分离 dA 方面表现出良好的性能，而在 2'-脱氧鸟苷（dG）、脱氧胞苷（dC）或 5'-单磷酸二钠盐（AMP）中则没有观察到印迹效应。此外，MMHSG 对 dA 的吸附遵循化学吸附，可在 60 分钟内达到吸附平衡；饱和吸附容量为 20.22 μmol·g。AcrU 的引入可以通过碱基互补配对提高选择性，从而使印迹因子大大提高到 3.79。因此，这是成功地将水凝胶与中空介孔硅纳米粒子和分子印迹材料结合在一起的一次尝试。
