MOE Key laboratory of Material Physics and Chemistry under Extraordinary Conditions, School of Science , Northwestern Polytechnical University , Xi'an 710072 , P. R. China.
ACS Appl Mater Interfaces. 2019 Jul 24;11(29):25682-25690. doi: 10.1021/acsami.9b06507. Epub 2019 Jul 9.
Protein imprinting technology is of interest in drug delivery, biosensing, solid-phase extraction, and so forth. However, the efficient recognition and separation of proteins have remained challenging to date. Toward this, under the assistance of Ni-bovine serum albumin (BSA) directional coordination strategy, magnetic BSA-imprinted materials had been synthesized via dopamine self-polymerization on hollow FeO@mSiO microspheres (mSiO referred as mesoporous silica). The well-defined imprinted microspheres possessed more satisfactory adsorption capacity (266.99 mg/g), enhanced imprinting factor (5.45), and fast adsorption saturation kinetics (40 min) for BSA, superior to many previous reports. Benefiting from the coordinate interaction between Ni and BSA, these fabricated microspheres exhibited excellent specificity not only in individual and competitive protein rebinding samples but also in bovine serum. Combined with the directional coordination method, the magnetic-imprinted composite materials to selectively capture target proteins could provide promising potential in applications.
蛋白质印迹技术在药物传递、生物传感、固相萃取等方面引起了人们的兴趣。然而，到目前为止，高效识别和分离蛋白质仍然具有挑战性。为此，在 Ni-牛血清白蛋白 (BSA) 定向配位策略的辅助下，通过多巴胺在中空 FeO@mSiO 微球（mSiO 表示介孔硅）上的自聚合，合成了磁性 BSA 印迹材料。这些具有良好定义的印迹微球具有更高的吸附容量（266.99mg/g）、增强的印迹因子（5.45）和快速的吸附饱和动力学（40 分钟），用于 BSA，优于许多先前的报道。由于 Ni 和 BSA 之间的配位相互作用，这些制备的微球不仅在单个和竞争蛋白质再结合样品中，而且在牛血清中都表现出优异的特异性。结合定向配位方法，磁性印迹复合材料可用于选择性捕获靶蛋白，在应用中具有广阔的前景。
