Ni-BSA Directional Coordination-Assisted Magnetic Molecularly Imprinted Microspheres with Enhanced Specific Rebinding to Target Proteins.

Protein imprinting technology is of interest in drug delivery, biosensing, solid-phase extraction, and so forth. However, the efficient recognition and separation of proteins have remained challenging to date. Toward this, under the assistance of Ni-bovine serum albumin (BSA) directional coordination strategy, magnetic BSA-imprinted materials had been synthesized via dopamine self-polymerization on hollow FeO@mSiO microspheres (mSiO referred as mesoporous silica). The well-defined imprinted microspheres possessed more satisfactory adsorption capacity (266.99 mg/g), enhanced imprinting factor (5.45), and fast adsorption saturation kinetics (40 min) for BSA, superior to many previous reports. Benefiting from the coordinate interaction between Ni and BSA, these fabricated microspheres exhibited excellent specificity not only in individual and competitive protein rebinding samples but also in bovine serum. Combined with the directional coordination method, the magnetic-imprinted composite materials to selectively capture target proteins could provide promising potential in applications.