Biotechnology of Reproduction, Department Clinics, Faculty of Veterinary Medicine, Iasi University of Life Sciences (IULS), M. Sadoveanu Alee, No 6, 700489 Iași, Romania.
Reproduction and Obstetrics, Department Clinics, Faculty of Veterinary Medicine, Iasi University of Life Sciences (IULS), M. Sadoveanu Alee, No 6, 700489 Iași, Romania.
Molecules. 2022 Nov 3;27(21):7539. doi: 10.3390/molecules27217539.
There is little information in the literature about the fungal contamination of boar semen and its persistence during storage. The challenge of this study was to perform a mycological screening to identify the yeast in the raw semen at 12/24 h after dilution. The research was done in pig farms in the N-E area of Romania, with maximum biosecurity and state-of-the-art technology. All the examined ejaculates (101) were considered to be normal for each spermogram parameter, with microbiological determinations in T0 at the time of ejaculate collection, T1 at the time of dilution, and T2 at 24 h of storage. Microbiological determinations (mycological spermogram) were performed for quantitative (LogCFU/mL) and qualitative (typification of fungal genera) identification. Bacterial burden (×103 LogCFU/mL) after dilution (T1) decreased drastically (p < 0.0001) compared to the one in the raw semen (T0). After 24 h of storage at 17 °C, the mean value of the bacteriospermia remained constant at an average value of 0.44. Mycospermia had a constant trend at T0 (raw) and T1 (0.149 vs. 0.140) and was slightly higher at T2 (0.236). The difference between T1 vs. T2 (p = 0.0419) was close to the statistical reference value (p = 0.05). Of the total genera identified (24), the fungi had a proportion of 37.4% (9/15) and a ratio of 1:1.6. Regarding the total species (34), the fungi had a frequency of 29.42% (10/24) with a ratio between the fungi and bacteria of 1:2.4. A fertility rate of 86% was observed in the L1 group (50 AI sows with doses and mycospermia from T1), and an 82% rate was observed in the L2 group (50 AI sows with doses and mycospermia from T2). The litter size of L1 was 9.63 piglets and 9.56 for L2. Regarding the total number of piglets obtained between the two groups, there was a slight decrease of 22 piglets in group L2, without statistical differences (p > 0.05). The predominant genera persisted after dilution during a 12 h storage at 17 °C, where yeasts, such as Candida parapsilosis and C. sake were identified in more than 92% of AI doses.
关于野猪精液的真菌污染及其在储存过程中的持续存在，文献中鲜有报道。本研究的挑战是在稀释后 12/24 小时对原始精液进行真菌筛查，以鉴定酵母。研究在罗马尼亚东北部的养猪场进行，采用最高水平的生物安全和最先进的技术。每个精子参数的检查精液（101 个）都被认为是正常的，在精液采集时的 T0、稀释时的 T1 和储存 24 小时时的 T2 进行微生物学测定。进行微生物学测定（真菌精子）进行定量（LogCFU/mL）和定性（真菌属的分类）鉴定。与原始精液（T0）相比，稀释后（T1）的细菌负荷（×103 LogCFU/mL）急剧下降（p < 0.0001）。在 17°C 下储存 24 小时后，菌血症的平均值保持不变，平均为 0.44。在 T0（原始）和 T1（0.149 对 0.140）时，真菌精子呈持续趋势，在 T2 时略高（0.236）。T1 与 T2 之间的差异（p = 0.0419）接近统计参考值（p = 0.05）。在鉴定的总属（24 个）中，真菌占 37.4%（9/15），比例为 1:1.6。在总种（34 种）中，真菌的频率为 29.42%（10/24），真菌与细菌的比例为 1:2.4。L1 组（50 头母猪配种剂量和 T1 时的真菌精子）的受精率为 86%，L2 组（50 头母猪配种剂量和 T2 时的真菌精子）的受精率为 82%。L1 的仔猪数为 9.63 头，L2 为 9.56 头。关于两组之间获得的仔猪总数，L2 组略有减少 22 头仔猪，但无统计学差异（p > 0.05）。在 17°C 下储存 12 小时期间，稀释后持续存在的主要属，鉴定出超过 92%的 AI 剂量中存在念珠菌属和清酒酵母等酵母。
