Saraeva Irina, Tolordava Eteri, Yushina Yulia, Sozaev Islam, Sokolova Vera, Khmelnitskiy Roman, Sheligyna Svetlana, Pallaeva Tatiana, Pokryshkin Nikolay, Khmelenin Dmitry, Ionin Andrey, Semenova Anastasia, Kudryashov Sergey
P. N. Lebedev Physics Institute, Russian Academy of Sciences, 119991 Moscow, Russia.
V. M. Gorbatov Federal Research Center for Food Systems, Russian Academy of Sciences, 109316 Moscow, Russia.
Nanomaterials (Basel). 2022 Nov 1;12(21):3857. doi: 10.3390/nano12213857.
We report the bactericidal effect of Ag and Cu NPs with different concentrations on methicillin-resistant strain in comparison to the effect of AgNO and CuCl solutions, characterized by microbiological tests, TEM and Fourier-transform infrared spectroscopy. NPs were produced by nanosecond laser ablation in distilled water and characterized by scanning electron microscopy, UV-vis, energy dispersive X-ray, FT-IR spectroscopy, as well as X-ray diffraction, dynamic light scattering size and zeta-potential measurements. Microbiological tests showed antibacterial activity of NPs and metal ion-containing salts. Comparative FT-IR spectroscopy of bacteria, treated with metal NPs and salts, showed the broadening of amide I and II bands, a CH-related peak and its frequency decrease, indicating the increase of membrane fluidity. The main mechanisms of the antibacterial effect were proposed: Ag and Cu NPs release ions and ROS, which result in lipid peroxidation; AgNO forms precipitates on the cell surface, which lead to the mechanical rupture of the membrane and subsequent possible penetration of the precipitates in the emerged damaged spots, complete destruction of the membrane and bacterial death; Cu ions from the CuCl solution cause damage to phosphorus- and sulfur-containing biomolecules, which leads to disruption of intracellular biochemical processes. The theories were confirmed by FT-IR spectroscopy and TEM.
我们报告了不同浓度的银纳米颗粒（Ag NPs）和铜纳米颗粒（Cu NPs）对耐甲氧西林菌株的杀菌效果，并与硝酸银（AgNO）溶液和氯化铜（CuCl）溶液的效果进行了比较，通过微生物学测试、透射电子显微镜（TEM）和傅里叶变换红外光谱（FT-IR）对其进行了表征。纳米颗粒通过纳秒激光烧蚀在蒸馏水中制备，并通过扫描电子显微镜、紫外可见光谱、能量色散X射线、傅里叶变换红外光谱以及X射线衍射、动态光散射尺寸和zeta电位测量对其进行了表征。微生物学测试显示了纳米颗粒和含金属离子盐的抗菌活性。用金属纳米颗粒和盐处理后的细菌的比较傅里叶变换红外光谱显示，酰胺I和II带变宽、一个与CH相关的峰及其频率降低，表明膜流动性增加。提出了抗菌作用的主要机制：银纳米颗粒和铜纳米颗粒释放离子和活性氧（ROS），导致脂质过氧化；硝酸银在细胞表面形成沉淀物，导致膜的机械破裂，随后沉淀物可能渗透到出现的损伤部位，膜完全破坏和细菌死亡；氯化铜溶液中的铜离子对含磷和含硫的生物分子造成损害，导致细胞内生化过程的破坏。这些理论通过傅里叶变换红外光谱和透射电子显微镜得到了证实。
