State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
School of Food Science and Technology, Nanchang University, Nanchang 330031, China.
Nutrients. 2022 Nov 7;14(21):4700. doi: 10.3390/nu14214700.
The reported association of seeds and allergic disease clinically resembling occupational asthma in cosmetic manufacturing workers has resultedin the need to identify such components in the manufacturing process. However, leaves from the same plant, an important food ingredient, have limited immunotoxicity data. This study aimed to determine if leafproteins (MLP) can elicit allergic responses in BALB/c mice. The BALB/c mice were sensitized twice and challenged 10 times to evaluate the potential allergenicityof MLP in vivo. The results showed increased levels of mast cells, total and specific IgE and IgG, severe signs of systemic anaphylaxis, and reduced body temperature compared with controls. The sensitized mice serum observed enhanced levels of histamine and Th-related cytokine release. Compared with the control group, increased levels of interleukins IL-4, IL-9, and IL-17A and enhanced expression and secretion of normal T cells were found in the culture supernatant of splenocytes treated with MLP.This study suggeststhat MLPcanelicit allergic responses; this providesmore comprehensive guidance for identifying new allergen candidates and developing hypoallergenic MLP products.
在化妆品制造工人中，报告的种子与类似于职业性哮喘的过敏疾病之间的关联，导致需要在制造过程中识别此类成分。然而，来自同一植物的叶子作为一种重要的食品成分，其免疫毒性数据有限。本研究旨在确定 叶蛋白（MLP）是否会在 BALB/c 小鼠中引发过敏反应。BALB/c 小鼠经两次致敏和 10 次攻击，以体内评估 MLP 的潜在变应原性。结果表明，与对照组相比，肥大细胞、总 IgE 和 IgG 水平升高，全身过敏症状严重，体温降低。与对照组相比，致敏小鼠血清中组胺和 Th 相关细胞因子释放增加。与对照组相比，用 MLP 处理的脾细胞培养上清液中发现白细胞介素 IL-4、IL-9 和 IL-17A 的水平升高，以及正常 T 细胞的表达和分泌增强。本研究表明 MLP 可引发过敏反应；这为识别新的变应原候选物和开发低变应原性 MLP 产品提供了更全面的指导。
