Win Aye Nwe, Sankhuan Darunmas, Chintakovid Watcharra, Supaibulwatana Kanyaratt
Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Sericulture Research and Development Center, Pyin-Oo-Lwin, Mandalay 05081, Myanmar.
Plants (Basel). 2022 Oct 26;11(21):2850. doi: 10.3390/plants11212850.
Different shoot/root micro-environments were investigated for growth performances and nutraceutical compounds in leaves of mulberry ( L.) transplants. Single-node segments were taken from seedling-grown pots of three cultivars: Myanmar large leaf (MLL), Myanmar medium leaf (MML), and C14. Transplant production was compared in soil, vermiculite (V), or the dynamic root floating technique (DRFT). The highest survival percentage of the transplants was obtained from V-system, and MLL showed a higher shoot/root formation over two tested cultivars. The MLL transplants grown in V-system under white LED light (445 and 554 nm) at 200 μmol·m·s gave a fresh weight with superior qualified transplants compared to other treatments. The bioactive compounds in leaves of MLL, MML, and C14 were analyzed using GC-MS after incubation with different LED spectra. Ethanol extracts of the leaves revealed that more than 50% of the bioactive compounds were fatty acids and conjugates and varied according to spectra and cultivar. Blue LED light (445 nm) induced the production of total phenolics, whereas white LED light favored the production of total proteins, soluble sugar, and biomass. The modified environments at the root and aerial zones significantly influenced the growth and biochemical parameters of transplants, and this applied technique can elevate useful functional ingredients of mulberry leaves.
研究了不同的地上部/根部微环境对桑树（L.）移栽苗生长性能及叶片中营养成分的影响。从三个品种（缅甸大叶（MLL）、缅甸中叶（MML）和C14）的盆栽实生苗上截取单节段。比较了在土壤、蛭石（V）或动态根系漂浮技术（DRFT）中移栽苗的培育情况。在V系统中移栽苗的成活率最高，且MLL在两个受试品种中表现出更高的地上部/根部形成率。在V系统中，于白色LED光（445和554 nm）、200 μmol·m·s条件下生长的MLL移栽苗，与其他处理相比，其鲜重更高且移栽苗质量更优。用不同LED光谱处理后，采用GC-MS分析MLL、MML和C14叶片中的生物活性化合物。叶片的乙醇提取物显示，超过50%的生物活性化合物为脂肪酸及其共轭物，且因光谱和品种而异。蓝色LED光（445 nm）诱导总酚类物质的产生，而白色LED光有利于总蛋白、可溶性糖和生物量的产生。根部和地上部区域的改良环境显著影响移栽苗的生长和生化参数，且这种应用技术可提高桑叶中有用功能成分的含量。
