Robert Koch Institute (RKI), Center for Biological Threats and Special Pathogens, German Reference Laboratory for Poxviruses, Berlin, Germany.
World Health Organization (WHO), Department of Epidemic and Pandemic Preparedness and Prevention, Geneva, Switzerland.
Euro Surveill. 2022 Nov;27(45). doi: 10.2807/1560-7917.ES.2022.27.45.2200816.
Before the international spread of monkeypox in May 2022, PCR kits for the detection of orthopoxviruses, and specifically monkeypox virus, were rarely available. Here we describe the evaluation of 11 recently developed commercially available PCR kits for the detection of monkeypox virus DNA. All tested kits are currently intended for research use only and clinical performance still needs to be assessed in more detail, but all were suitable for diagnostics of monkeypox virus, with variations in specificity rather than sensitivity.
在 2022 年 5 月猴痘病毒在国际间传播之前，用于检测正痘病毒（特别是猴痘病毒）的 PCR 试剂盒很少见。在此，我们描述了对 11 种最近开发的商业上可获得的用于检测猴痘病毒 DNA 的 PCR 试剂盒的评估。所有经过测试的试剂盒目前仅用于研究用途，其临床性能仍需更详细地评估，但它们都适用于猴痘病毒的诊断，特异性方面存在差异，而不是敏感性。
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