Department of Oral Histology-Developmental Biology & Dental Research Institute, Laboratory for the Study of Regenerative Dental Medicine, School of Dentistry, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea.
HysensBio, Co., Ltd., 10 Dwitgol-ro, Gwacheon-si, Gyeonggi-do, Republic of Korea.
BMC Oral Health. 2022 Nov 11;22(1):483. doi: 10.1186/s12903-022-02558-8.
Dentin hypersensitivity is a painful response to external stimuli applied to exposed dentinal tubules. Various toothpastes with active desensitizing ingredients for the relief of dentin hypersensitivity are commercially available. However, data from several studies suggest that the effects of desensitizing toothpastes are unstable and brief. This study aimed to investigate the effect of toothpastes containing CPNE7-derived oligopeptide (CPNE7-DP) and other active desensitizing ingredients in the dentin microleakage, tubule occlusion and tertiary dentin formation.
Using scanning electron microscopy (SEM), we evaluated the patency of dentinal tubules on the surface of human dentin disks after brushing experiments with the various toothpastes. Dentin was histologically evaluated in a hypersensitivity model of canine teeth, after the exposed dentin area was brushed for 6 weeks. The toothpaste used in group 1 (control) did not contain any desensitizing ingredients; that used in group 2 contained CPNE7-DP; Colgate Sensitive was used in group 3; and Sensodyne Rapid Relief was used in group 4. Finally, we conducted microleakage analysis to investigate the dentin sealing effect. The microleakage analysis data were subjected to one-way ANOVA and post-hoc Tukey tests (alpha = 0.05).
In the SEM images, all four groups of teeth exhibited partial occlusion of the dentinal tubules on the tooth surface. In the in vivo hypersensitivity model, group 2 exhibited a newly formed tertiary dentin, whereas no new hard tissue formation was observed in groups 1, 3, and 4. Microleakage analysis revealed that the volume of dentinal fluid flow was significantly smaller in group 2 than in group 1.
These results indicate that CPNE7-DP is a promising active ingredient with long-term dentin sealing effects.
牙本质敏感是一种对外界刺激作用于暴露的牙本质小管的疼痛反应。市售的各种含有主动脱敏成分的牙膏都可以缓解牙本质敏感。然而，几项研究的数据表明，脱敏牙膏的效果不稳定且短暂。本研究旨在探讨含有 CPNE7 衍生寡肽（CPNE7-DP）和其他主动脱敏成分的牙膏对牙本质微渗漏、小管阻塞和第三期牙本质形成的影响。
使用扫描电子显微镜（SEM）评估各种牙膏刷牙实验后牙本质小管在牙本质圆盘表面的通畅性。在犬牙敏感模型中，对暴露的牙本质区域进行 6 周刷牙后，对牙本质进行组织学评估。组 1（对照组）使用的牙膏不含任何脱敏成分；组 2 含有 CPNE7-DP；组 3 使用高露洁敏感牙膏；组 4 使用舒适达速效抗敏牙膏。最后，我们进行了微渗漏分析以研究牙本质密封效果。微渗漏分析数据采用单因素方差分析和事后 Tukey 检验（alpha=0.05）。
在 SEM 图像中，四组牙齿的牙面均显示部分牙本质小管闭塞。在体内敏感模型中，组 2 表现出新生第三期牙本质，而组 1、组 3 和组 4 未观察到新的硬组织形成。微渗漏分析显示，组 2 的牙本质液流量明显小于组 1。
这些结果表明 CPNE7-DP 是一种有前途的具有长期牙本质密封效果的活性成分。
