Nakamura Y, Okamoto K, Tanimura A, Kato M, Morimatsu M
Cancer. 1986 Sep 1;58(5):1070-5. doi: 10.1002/1097-0142(19860901)58:5<1070::aid-cncr2820580515>3.0.co;2-6.
Elastin from both elastofibroma and control skin samples was analyzed by means of pancreatic elastase digestion and subsequent biochemical studies, such as for elastin content and amino acid composition. Elastin of elastofibroma was more resistant to elastase digestion than that of controls. Elastin content was increased almost twice (wet weight) or three times (dry weight) in elastofibroma. The amino acid composition had the characteristics of elastin; however, the cross-linking amino acids such as desmosine, isodesmosine, and lysinonorleucine were increased in elastofibroma when compared with controls. An electron microscopic study showed that the interspersed cells had prominent intermediate filaments without any periodicity, pinocytotic vesicles, rough endoplasmic reticulum, and other organelles. These cells were considered to be fibroblasts not myofibroblasts. Therefore, it could be supposed that fibroblasts newly form an elastin that has a slightly different amino acid composition from that of controls.
通过胰弹性蛋白酶消化及随后的生化研究，如弹性蛋白含量和氨基酸组成分析，对弹力纤维瘤和对照皮肤样本中的弹性蛋白进行了分析。弹力纤维瘤的弹性蛋白比对照样本的弹性蛋白对弹性蛋白酶消化更具抗性。弹力纤维瘤中弹性蛋白含量几乎增加了两倍（湿重）或三倍（干重）。氨基酸组成具有弹性蛋白的特征；然而，与对照相比，弹力纤维瘤中交联氨基酸如锁链素、异锁链素和赖氨酰正亮氨酸有所增加。电子显微镜研究显示，散在的细胞有突出的中间丝，无任何周期性，有吞饮小泡、粗面内质网和其他细胞器。这些细胞被认为是成纤维细胞而非肌成纤维细胞。因此，可以推测成纤维细胞新形成了一种氨基酸组成与对照略有不同的弹性蛋白。
