All-Russia Research Institute of Agricultural Biotechnology, Moscow, Russia.
N.V. Tsitsin Main Botanical Garden of the RAS, Moscow, Russia.
Planta. 2022 Nov 14;256(6):116. doi: 10.1007/s00425-022-04032-9.
StLFY-knockout potato plants were developed using CRISPR/Cas9 system. Inflorescences of edited plants transited to flowering, but inflorescence structures lacked flowers and were indeterminate, producing multiple shoot meristems. The tetraploid potato (Solanum tuberosum L.) is an important agricultural crop worldwide. In this study, we used CRISPR/Cas9 to inactivate the potato homolog (StLFY) of the LEAFY gene-a key regulator of the transition to flowering and floral meristem identity-in a tetraploid potato cultivar. We achieved high rates of all-allelic knockouts. Frameshift indels led to phenotypic alterations, including indeterminate inflorescence development and the replacement of flowers with the leafy-like structures.
利用 CRISPR/Cas9 系统培育 StLFY 敲除马铃薯植株。编辑植株的花序过渡到开花，但花序结构缺乏花朵且不定型，产生多个茎尖分生组织。四倍体马铃薯（Solanum tuberosum L.）是世界范围内重要的农业作物。在本研究中，我们使用 CRISPR/Cas9 使四倍体马铃薯品种中 LEAFY 基因的马铃薯同源物（StLFY）失活，该基因是向开花和花分生组织身份转变的关键调节因子。我们实现了全等位基因敲除的高成功率。移码缺失导致表型改变，包括不定型花序发育和有叶状结构替代花朵。
