College of Plant Protection, Southwest University, Chongqing 400715, China.
College of Plant Protection, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory of Soft-Matter Material Chemistry and Function Manufacturing, Southwest University, Chongqing 400715, China.
Int J Biol Macromol. 2022 Dec 31;223(Pt A):1208-1222. doi: 10.1016/j.ijbiomac.2022.11.072. Epub 2022 Nov 12.
Although ε-poly-l-lysine (ε-PL) has a good potential as a green fungicide, high concentration is usually required during its controlling of plant disease. On the other hand, same problems also appeared in the study of CuONP based nano pesticides. In this manuscript, a new composite alginate nanogel (ALGNP) that containing CuONP and ε-PL was fabricated via in situ reduction of CuONP in nanogel and ε-PL surface coating. Based on the chelation of amide bond of ε-PL and Cu released by CuONP, the synergy effect between Cu and ε-PL layer of the nanogel make the nanogel (CuONP@ALGNP@PL) performed high anti-fungal activity under low Cu and ε-PL concentration (Cu concentration was 40.09 μg/mL, ε-PL concentration was 11.90 μg/mL). Study showed that the nanogel could more significantly destroy the fungal cell membrane than CuONP@ALGNP and ALGNP@PL, also better than commercial fungicide CuCaSO (Cu concentration was 120 μg/mL). Furthermore, CuONP@ALGNP@PL could seriously affect the spore production, spore germination rate and bud tube elongation length of Alternaria alternate. Moreover, CuONP@ALGNP@PL also inhibit Botrytis cinerea, Phytophthora, Thanatephorus cucumeris and Fusarium graminearum. These results showed that composite of CuONP and ε-PL based on nanogel can decrease the raw materials application amount, and achieve a high disease controlling ability, which provides a new perspective for preventing fungal diseases.
虽然 ε-聚赖氨酸（ε-PL）作为一种绿色杀菌剂具有良好的应用潜力，但在其防治植物病害时通常需要高浓度使用。另一方面，在基于 CuONP 的纳米农药的研究中也出现了同样的问题。在本手稿中，通过在纳米凝胶中还原 CuONP 和在 ε-PL 表面涂覆，制备了一种新型的含 CuONP 和 ε-PL 的复合海藻酸钠纳米凝胶（ALGNP）。基于 ε-PL 酰胺键与 CuONP 释放的 Cu 的螯合作用，纳米凝胶中 Cu 和 ε-PL 层之间的协同作用使纳米凝胶（CuONP@ALGNP@PL）在低 Cu 和 ε-PL 浓度下（Cu 浓度为 40.09μg/mL，ε-PL 浓度为 11.90μg/mL）表现出高抗真菌活性。研究表明，与 CuONP@ALGNP 和 ALGNP@PL 相比，纳米凝胶能更显著地破坏真菌细胞膜，也优于商业杀菌剂 CuCaSO（Cu 浓度为 120μg/mL）。此外，CuONP@ALGNP@PL 能严重影响链格孢Alternaria alternate的孢子产生、孢子萌发率和芽管伸长长度。而且，CuONP@ALGNP@PL 还能抑制灰葡萄孢菌、疫霉属、黄瓜黑星病菌和禾谷镰刀菌。这些结果表明，基于纳米凝胶的 CuONP 和 ε-PL 复合材料可以减少原材料的使用量，并实现高的病害防治能力，为防治真菌病害提供了新的视角。
