College of Plant Protection, Southwest University, Chongqing 400715, China; Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, Ministry of Education, Chongqing 400715, China.
College of Plant Protection, Southwest University, Chongqing 400715, China.
Pestic Biochem Physiol. 2024 Jun;202:105959. doi: 10.1016/j.pestbp.2024.105959. Epub 2024 May 14.
ε-Poly-l-lysine (ε-PL) is an effective antimicrobial peptide for controlling fungal plant diseases, exhibiting significant antifungal activity and safety. Despite its known efficacy, the potential of ε-PL in combating plant bacterial diseases remains underexplored. This study evaluated the effectiveness of ε-PL and its nanomaterial derivative in managing tomato bacterial spot disease caused by Pseudomonas syringae pv. tomato. Results indicated that ε-PL substantially inhibited the growth of Pseudomonas syringae pv. tomato. Additionally, when ε-PL was loaded onto attapulgite (encoded as ATT@PL), its antibacterial effect was significantly enhanced. Notably, the antibacterial efficiency of ATT@PL containing 18.80 μg/mL ε-PL was even close to that of 100 μg/mL pure ε-PL. Further molecular study results showed that, ATT@PL stimulated the antioxidant system and the salicylic acid signaling pathway in tomatoes, bolstering the plants disease resistance. Importantly, the nanocomposite demonstrated no negative effects on both seed germination and plant growth, indicating its safety and aligning with sustainable agricultural practices. This study not only confirmed the effectiveness of ε-PL in controlling tomato bacterial spot disease, but also introduced an innovative high antibacterial efficiency ε-PL composite with good bio-safety. This strategy we believe can also be used in improving other bio-pesticides, and has high applicability in agriculture practice.
ε-聚赖氨酸（ε-PL）是一种有效的抗真菌肽，可用于控制植物真菌病害，具有显著的抗真菌活性和安全性。尽管其功效已被广泛认知，但 ε-PL 在防治植物细菌性病害方面的潜力尚未得到充分探索。本研究评估了 ε-PL 及其纳米材料衍生物在防治由丁香假单胞菌 pv.番茄引起的番茄细菌性斑点病中的效果。结果表明，ε-PL 可显著抑制丁香假单胞菌 pv.番茄的生长。此外，当 ε-PL 负载到凹凸棒石（编码为 ATT@PL）上时，其抗菌效果显著增强。值得注意的是，含 18.80μg/mL ε-PL 的 ATT@PL 的抗菌效率甚至接近 100μg/mL 纯 ε-PL 的抗菌效率。进一步的分子研究结果表明，ATT@PL 刺激了番茄中的抗氧化系统和水杨酸信号通路，增强了植物的抗病性。重要的是，该纳米复合材料对种子萌发和植物生长均无不良影响，表明其安全性符合可持续农业实践。本研究不仅证实了 ε-PL 控制番茄细菌性斑点病的有效性，还引入了一种具有良好生物安全性的新型高抗菌效率 ε-PL 复合材料。我们相信，这种策略还可以用于改进其他生物农药，并在农业实践中具有很高的适用性。
