Department of Botany and Microbiology, Faculty of Science, Sohag University, Sohag 82524, Egypt.
Department of Botany and Microbiology, Faculty of Science, Assuit University, Assiut 71516, Egypt.
J Microbiol Biotechnol. 2022 Dec 28;32(12):1537-1546. doi: 10.4014/jmb.2208.08004. Epub 2022 Nov 1.
is a cause of high mortality in humans and therefore it is necessary to prevent its transmission and reduce infections. Our goals in this research were to investigate the frequency of methicillin-resistant (MRSA) in Taif, Saudi Arabia, and assess the relationship between the phenotypic antimicrobial sensitivity patterns and the genes responsible for resistance. In addition, we examined the antimicrobial efficiency and application of silver nanoparticles (AgNPs) against MRSA isolates. Seventy-two nasal swabs were taken from patients; MRSA was cultivated on Mannitol Salt Agar supplemented with methicillin, and 16S rRNA sequencing was conducted in addition to morphological and biochemical identification. Specific resistance genes such as , , , and were PCR-amplified and resistance plasmids were also investigated. The MRSA incidence was ~49 % among the 72 isolates and all MRSA strains were resistant to oxacillin, penicillin, and cefoxitin. However, vancomycin, linezolid, teicoplanin, mupirocin, and rifampicin were effective against 100% of MRSA strains. About 61% of MRSA strains exhibited multidrug resistance and were resistant to 3-12 antimicrobial medications (MDR). Methicillin resistance gene mecA was presented in all MDR-MRSA strains. Most MDR-MRSA contained a plasmid of > 10 kb. To overcome bacterial resistance, AgNPs were applied and displayed high antimicrobial activity and synergistic effect with penicillin. Our findings may help establish programs to control bacterial spread in communities as AgNPs appeared to exert a synergistic effect with penicillin to control bacterial resistance.
金黄色葡萄球菌是导致人类高死亡率的原因之一，因此有必要预防其传播并减少感染。我们在这项研究中的目标是调查沙特阿拉伯塔伊夫市耐甲氧西林金黄色葡萄球菌（MRSA）的频率，并评估表型抗微生物敏感性模式与耐药基因之间的关系。此外，我们还研究了银纳米粒子（AgNPs）对 MRSA 分离株的抗菌效率和应用。从患者中采集了 72 个鼻拭子；在添加了甲氧西林的甘露醇盐琼脂上培养了 MRSA，并进行了 16S rRNA 测序以及形态学和生化鉴定。此外，还扩增了特定的耐药基因，如 、 、 、 ，并研究了耐药质粒。在 72 株分离株中，MRSA 的发生率约为 49%，所有 MRSA 菌株均对苯唑西林、青霉素和头孢西丁耐药。然而，万古霉素、利奈唑胺、替考拉宁、莫匹罗星和利福平对 100%的 MRSA 菌株有效。约 61%的 MRSA 菌株表现出多药耐药性，对 3-12 种抗菌药物（MDR）耐药。所有 MDR-MRSA 菌株均携带 mecA 耐药基因。大多数 MDR-MRSA 含有>10kb 的质粒。为了克服细菌耐药性，应用了 AgNPs，显示出高抗菌活性，并与青霉素具有协同作用。我们的研究结果可能有助于建立控制社区中细菌传播的计划，因为 AgNPs 似乎与青霉素具有协同作用，可以控制细菌耐药性。
