Hasapis Kyriacos A, Charalambidou Iris, Tsouma Eleni, Sotiriadi Konstantina, Kassinis Nicolaos, Schou Chad, Karanis Panagiotis
University of Nicosia Medical School, Department of Basic and Clinical Sciences, 21 Ilia Papakyriakou, 2414, P.O. Box 24005, 1700, Engomi, Nicosia, Cyprus.
University of Nicosia, Department of Life and Health Sciences, School of Sciences and Engineering, Nicosia, Cyprus.
Parasitol Res. 2023 Jan;122(1):201-205. doi: 10.1007/s00436-022-07717-y. Epub 2022 Nov 17.
Cryptosporidium is an important intestinal zoonotic pathogen that can infect various hosts and cause diarrheal disease. There are no reports on the prevalence and molecular characterization of Cryptosporidium in wild birds in Cyprus. Therefore, the present study aimed to determine the prevalence of Cryptosporidium spp. and genotypes in wild birds found at Phassouri Reedbeds (Akrotiri Wetlands), Cyprus. Fecal samples of 75 wild birds (Eurasian coot Fulica atra, N = 48; Eurasian teal Anas crecca, N = 20; duck - Anas spp., Ν = 7) were screened for Cryptosporidium by PCR amplification and sequencing. Only one sample (1.3%) belonging to a Eurasian coot was PCR-positive for Cryptosporidium. Based on sequencing of the 18S rRNA locus, this species was identified as Cryptosporidium proventriculi. This is the first report on the molecular identification of this Cryptosporidium species in a Eurasian coot.
隐孢子虫是一种重要的肠道人畜共患病原体，可感染多种宿主并引起腹泻病。目前尚无关于塞浦路斯野生鸟类中隐孢子虫流行情况和分子特征的报道。因此，本研究旨在确定塞浦路斯法苏里芦苇床（阿克罗蒂里湿地）发现的野生鸟类中隐孢子虫种类和基因型的流行情况。通过PCR扩增和测序对75只野生鸟类（白骨顶鸡，N = 48；绿翅鸭，N = 20；鸭属，N = 7）的粪便样本进行隐孢子虫筛查。仅一份属于白骨顶鸡的样本（1.3%）对隐孢子虫PCR检测呈阳性。基于18S rRNA基因座的测序，该物种被鉴定为前胃隐孢子虫。这是关于该隐孢子虫种类在白骨顶鸡中分子鉴定的首次报道。
