An Dong, Song Liyun, Li Ying, Shen Lili, Miao Pu, Wang Yujie, Liu Dongyang, Jiang Lianqiang, Wang Fenglong, Yang Jinguang
Key Laboratory of Tobacco Pest Monitoring, Controlling and Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China.
Luoyang City Company of Henan Province Tobacco Company, Luoyang, China.
Front Genet. 2022 Nov 1;13:1014225. doi: 10.3389/fgene.2022.1014225. eCollection 2022.
Western flower thrips () are among the most important pests globally that transmit destructive plant viruses and infest multiple commercial crops. Lysine lactylation (Klac) is a recently discovered novel post-translational modification (PTM). We used liquid chromatography-mass spectrometry to identify the global lactylated proteome of , and further enriched the identified lactylated proteins using Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO). In the present study, we identified 1,458 Klac sites in 469 proteins from . Bioinformatics analysis showed that Klac was widely distributed in proteins, and these Klac modified proteins participated in multiple biological processes. GO and KEGG enrichment analysis revealed that Klac proteins were significantly enriched in multiple cellular compartments and metabolic pathways, such as the ribosome and carbon metabolism pathways. Two Klac proteins were found to be involved in the regulation of the TSWV (Tomato spotted wilt virus) transmission in . This study provides a systematic report and a rich dataset of lactylation in proteome for potential studies on the Klac protein of this notorious pest.
西花蓟马是全球最重要的害虫之一，可传播具有破坏性的植物病毒，并侵害多种经济作物。赖氨酸乳酰化（Klac）是最近发现的一种新型翻译后修饰（PTM）。我们使用液相色谱-质谱法鉴定了西花蓟马的全局乳酰化蛋白质组，并利用京都基因与基因组百科全书（KEGG）和基因本体论（GO）进一步富集了鉴定出的乳酰化蛋白质。在本研究中，我们从西花蓟马中鉴定出469种蛋白质中的1458个Klac位点。生物信息学分析表明，Klac在西花蓟马蛋白质中广泛分布，这些Klac修饰的蛋白质参与多种生物学过程。GO和KEGG富集分析显示，Klac蛋白质在多个细胞区室和代谢途径中显著富集，如核糖体和碳代谢途径。发现两种Klac蛋白质参与西花蓟马中番茄斑萎病毒（TSWV）传播的调控。本研究为这种臭名昭著的害虫的Klac蛋白质的潜在研究提供了一份关于西花蓟马蛋白质组中乳酰化的系统报告和丰富数据集。
