Zhang Xuhong, Han Changzhi, Liang Yuqin, Yang Yang, Liu Yun, Cao Yanpo
Institute of Cash Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, China.
Landscape Management and Protection Center, Shijiazhuang Bureau of Landscape Architecture, Shijiazhuang, China.
Front Plant Sci. 2022 Oct 27;13:1050840. doi: 10.3389/fpls.2022.1050840. eCollection 2022.
Soil salinity is a very serious abiotic stressor that affects plant growth and threatens crop yield. Thus, it is important to explore the mechanisms of salt tolerance of plant and then to stabilize and improve crop yield. Asparagus is an important cash crop, but its salt tolerance mechanisms are largely unknown. Full-length transcriptomic and metabolomic analyses were performed on two asparagus genotypes: 'jx1502' (a salt-tolerant genotype) and 'gold crown' (a salt-sensitive genotype). Compared with the distilled water treatment (control), 877 and 1610 differentially expressed genes (DEGs) were identified in 'jx1502' and 'gold crown' under salt stress treatment, respectively, and 135 and 73 differentially accumulated metabolites (DAMs) were identified in 'jx1502' and 'gold crown' under salt stress treatment, respectively. DEGs related to ion transport, plant hormone response, and cell division and growth presented differential expression profiles between 'jx1502' and 'gold crown.' In 'jx1502,' 11 ion transport-related DEGs, 8 plant hormone response-related DEGs, and 12 cell division and growth-related DEGs were upregulated, while 7 ion transport-related DEGs, 4 plant hormone response-related DEGs, and 2 cell division and growth-related DEGs were downregulated. Interestingly, in 'gold crown,' 14 ion transport-related DEGs, 2 plant hormone response-related DEGs, and 6 cell division and growth-related DEGs were upregulated, while 45 ion transport-related DEGs, 13 plant hormone response-related DEGs, and 16 cell division and growth-related DEGs were downregulated. Genotype 'jx1502' can modulate K/Na and water homeostasis and maintain a more constant transport system for nutrient uptake and distribution than 'gold crown' under salt stress. Genotype 'jx1502' strengthened the response to auxin (IAA), as well as cell division and growth for root remodeling and thus salt tolerance. Therefore, the integration analysis of transcriptomic and metabolomic indicated that 'jx1502' enhanced sugar and amino acid metabolism for energy supply and osmotic regulatory substance accumulation to meet the demands of protective mechanisms against salt stress. This work contributed to reveal the underlying salt tolerance mechanism of asparagus at transcription and metabolism level and proposed new directions for asparagus variety improvement.
土壤盐渍化是一种非常严重的非生物胁迫因素，影响植物生长并威胁作物产量。因此，探索植物的耐盐机制进而稳定和提高作物产量具有重要意义。芦笋是一种重要的经济作物，但其耐盐机制在很大程度上尚不清楚。对两种芦笋基因型：“jx1502”（耐盐基因型）和“金冠”（盐敏感基因型）进行了全长转录组和代谢组分析。与蒸馏水处理（对照）相比，在盐胁迫处理下，“jx1502”和“金冠”中分别鉴定出877个和1610个差异表达基因（DEG），在盐胁迫处理下，“jx1502”和“金冠”中分别鉴定出135个和73个差异积累代谢物（DAM）。与离子运输、植物激素反应以及细胞分裂和生长相关的DEG在“jx1502”和“金冠”之间呈现出不同的表达谱。在“jx1502”中，11个与离子运输相关的DEG、8个与植物激素反应相关的DEG和12个与细胞分裂和生长相关的DEG上调，而7个与离子运输相关的DEG、4个与植物激素反应相关的DEG和2个与细胞分裂和生长相关的DEG下调。有趣的是，在“金冠”中，14个与离子运输相关的DEG、2个与植物激素反应相关的DEG和6个与细胞分裂和生长相关的DEG上调，而45个与离子运输相关的DEG、13个与植物激素反应相关的DEG和16个与细胞分裂和生长相关的DEG下调。基因型“jx1502”在盐胁迫下比“金冠”能调节钾/钠和水分稳态，并维持更稳定的养分吸收和分配运输系统。基因型“jx1502”增强了对生长素（IAA）的反应，以及细胞分裂和生长以进行根系重塑，从而提高耐盐性。因此，转录组和代谢组的整合分析表明，“jx1502”增强了糖和氨基酸代谢以提供能量供应和积累渗透调节物质，以满足抵御盐胁迫保护机制的需求。这项工作有助于在转录和代谢水平揭示芦笋潜在的耐盐机制，并为芦笋品种改良提出新的方向。
