State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, PR China.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, PR China.
Anal Chim Acta. 2022 Dec 15;1236:340576. doi: 10.1016/j.aca.2022.340576. Epub 2022 Nov 2.
Listeria monocytogenes (L. monocytogenes), a typical foodborne pathogen, poses a serious threat to public health safety. This stimulates to develop a point-of-care testing (POCT) method to achieve rapid, sensitive detection of L. monocytogenes. In this study, polyethylene glycol (PEG) mediated ampicillin functionalized magnetic beads (Amp-PEG-MBs) was prepared successfully and it achieved high efficiency (>90%) and rapid (5 min) capture for L. monocytogenes at room temperature. The innovative combination of antibody (Ab), glucose oxidase (GOD) and graphene oxide (GO) prepared Ab@GO@GOD for the specific recognition of L. monocytogenes. Finally, Amp-PEG-MBs and Ab@GO@GOD were successfully assembled into Amp-PEG-MBs@L. monocytogenes-Ab@GO@GOD sandwich structure which could catalyze the glucose, and the final detection results were recorded by a blood glucose meter (BGM). Magnetic separation (MS) combined with enzyme-catalyzed sensor (MS-Ab@GO@GOD-BGM) was successfully established to achieve the detection of L. monocytogenes in artificially contaminated juice within 66 min with the limit of detection was 10 CFU/mL. This sensor has potential for other pathogens detection by modifying specific antibodies.
李斯特菌（Listeria monocytogenes，L. monocytogenes）是一种典型的食源性致病菌，对公共卫生安全构成严重威胁。这激发了人们开发即时检测（POCT）方法，以实现李斯特菌的快速、灵敏检测。本研究成功制备了聚乙二醇（PEG）介导的氨苄青霉素功能化磁性珠（Amp-PEG-MBs），在室温下对李斯特菌实现了高效（>90%）和快速（5 min）捕获。将抗体（Ab）、葡萄糖氧化酶（GOD）和氧化石墨烯（GO）进行创新性组合，制备了 Ab@GO@GOD 用于李斯特菌的特异性识别。最后，Amp-PEG-MBs 和 Ab@GO@GOD 成功组装成 Amp-PEG-MBs@L. monocytogenes-Ab@GO@GOD 三明治结构，该结构可以催化葡萄糖，最终的检测结果由血糖仪（BGM）记录。通过磁分离（MS）和酶催化传感器（MS-Ab@GO@GOD-BGM）的结合，成功建立了用于在 66 min 内检测人工污染果汁中李斯特菌的方法，检测限为 10 CFU/mL。通过修饰特异性抗体，该传感器具有用于其他病原体检测的潜力。
