Elliott S D, Tai J Y
J Exp Med. 1978 Dec 1;148(6):1699-704. doi: 10.1084/jem.148.6.1699.
Streptococcus suis types 1 and 2 were subjected to digestion with lysozyme. Serologically type-specific capsular polysaccharides were isolated from the lysates by ethanol precipitation followed by Sepharose 6B chromatography. The purified type 1 polysaccharide has a Kd value of 0.074 on a Sepharose 4B column and contains galactose, glucose, N-acetyl glucosamine, N-acetyl galactosamine, and sialic acid in a molar ratio of 2.42:1.00:1.00:1.13:1.39. The type 2 polysaccharide has a Kd value of 0.185 and is composed of rhamnose, galactose, glucose, N-acetyl glucosamine, and sialic acid in a molar ratio of 1.07:3.17:1.00:0.94:1.00. A comparison is drawn between the type polysaccharides of S. suis and those of group B streptococci.
对1型和2型猪链球菌进行溶菌酶消化处理。通过乙醇沉淀，随后进行琼脂糖6B柱色谱法，从裂解物中分离出血清学上型特异性的荚膜多糖。纯化的1型多糖在琼脂糖4B柱上的Kd值为0.074，含有半乳糖、葡萄糖、N - 乙酰葡糖胺、N - 乙酰半乳糖胺和唾液酸，其摩尔比为2.42:1.00:1.00:1.13:1.39。2型多糖的Kd值为0.185，由鼠李糖、半乳糖、葡萄糖、N - 乙酰葡糖胺和唾液酸组成，摩尔比为1.07:3.17:1.00:0.94:1.00。对猪链球菌的型多糖与B组链球菌的型多糖进行了比较。
