Institute of Analytical and Bioanalytical Chemistry, Ulm University, Albert Einstein-Allee 11, 89081, Ulm, Germany.
Institute of Pharmacology and Toxicology, Ulm University Medical Center, Albert Einstein-Allee 11, 89081, Ulm, Germany.
NPJ Biofilms Microbiomes. 2022 Nov 19;8(1):92. doi: 10.1038/s41522-022-00353-5.
Foodborne pathogenic microorganisms form biofilms at abiotic surfaces, which is a particular challenge in food processing industries. The complexity of biofilm formation requires a fundamental understanding on the involved molecular mechanisms, which may then lead to efficient prevention strategies. In the present study, biogenic amine producing bacteria, i.e., Lentilactobacillus parabuchneri DSM 5987 strain isolated from cheese were studied in respect with biofilm formation, which is of substantial relevance given their contribution to the presence of histamine in dairy products. While scanning electron microscopy was used to investigate biofilm adhesion at stainless steel surfaces, in situ infrared attenuated total reflection spectroscopy (IR-ATR) using a custom flow-through assembly was used for real-time and non-destructive observations of biofilm formation during a period of several days. The spectral window of 1700-600 cm provides access to vibrational signatures characteristic for identifying and tracking L. parabuchneri biofilm formation and maturation. Especially, the amide I and II bands, lactic acid produced as the biofilm matures, and a pronounced increase of bands characteristic for extracellular polymeric substances (EPS) provide molecular insight into biofilm formation, maturation, and changes in biofilm architecture. Finally, multivariate data evaluation strategies were applied facilitating the unambiguous classification of the observed biofilm changes via IR spectroscopic data.
食源性病原体在非生物表面形成生物膜，这对食品加工行业来说是一个特别的挑战。生物膜形成的复杂性要求对所涉及的分子机制有一个基本的了解，这可能会导致有效的预防策略。在本研究中，从奶酪中分离出的植物乳杆菌 DSM 5987 菌株等生物胺产生菌在生物膜形成方面进行了研究，鉴于其对乳制品中组胺存在的贡献，这具有重要意义。虽然扫描电子显微镜用于研究不锈钢表面的生物膜附着，但原位红外衰减全反射光谱（IR-ATR）使用定制的流动通过组件用于在几天的时间内对生物膜形成进行实时和非破坏性观察。1700-600 cm 的光谱窗口可用于识别和跟踪 L. parabuchneri 生物膜形成和成熟的特征振动特征。特别是酰胺 I 和 II 带、生物膜成熟时产生的乳酸，以及特征性的细胞外聚合物（EPS）带的明显增加，为生物膜形成、成熟和生物膜结构变化提供了分子见解。最后，应用了多元数据分析策略，通过红外光谱数据方便地对观察到的生物膜变化进行了明确的分类。
