Cardiovascular Disease Center, The Affiliated Suzhou Hospital of Nanjing Medical University, Jiangsu , 215008, Suzhou, P.R. China.
Department of Ultrasonography, The Fourth Affiliated Hospital of Anhui Medical University, 23000, Hefei, Anhui, P.R. China.
BMC Pregnancy Childbirth. 2022 Nov 19;22(1):853. doi: 10.1186/s12884-022-05190-0.
Tetralogy of fallot (TOF) in the fetus is a typical congential heart disease that occurs during the early embryonic period, being characterized by the abnormal development of conus arteriosus. The early diagnosis and prevention of fetal TOF is very important and there is a great need for exploring the pathogenesis of it in clinic. In this study, there were three cases being detected with TOF by fetal echocardiogram and confirmed by autopsy. We characterize the difference of expression of lncRNAs and mRNAs through sequencing analysis of 3 pairs of myocardial tissues of fetal TOF and those of age-matched controls. Compared with normal group, there were 94 differentially expressed lncRNAs and 83 mRNA transcripts in TOF (P < 0.05). Correlation analysis between lncRNA and mRNA further showed that differentially expressed lncRNA can be linked to mRNAs, suggesting the potential regulator role of lncRNA in mRNA expression. Our data serve as a fundamental resource for understanding the disease etiology of TOF.
法洛四联症（TOF）是一种典型的先天性心脏病，发生在胚胎早期，其特征是动脉圆锥发育异常。胎儿 TOF 的早期诊断和预防非常重要，临床上迫切需要探索其发病机制。本研究通过胎儿超声心动图检测到 3 例 TOF，并通过尸检证实。我们通过对 3 对胎儿 TOF 心肌组织和年龄匹配的对照组心肌组织的测序分析，描述了长链非编码 RNA 和信使 RNA 表达的差异。与正常组相比，TOF 组有 94 个差异表达的 lncRNA 和 83 个 mRNA 转录本（P < 0.05）。lncRNA 与 mRNA 之间的相关性分析进一步表明，差异表达的 lncRNA 可以与 mRNAs 相关联，提示 lncRNA 在 mRNA 表达中可能具有调节作用。我们的数据为了解 TOF 的疾病病因提供了基础资源。
