Department of Gastroenterology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China.
Department of Gastrointestinal Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China.
Cancer Sci. 2023 Mar;114(3):1075-1085. doi: 10.1111/cas.15661. Epub 2022 Nov 29.
Gastric cancer (GC) is one of the most common malignant tumors with a high incidence and mortality. Microbiota play a significant role in human health and disease. We aimed to investigate the prognostic value of the gastric microbiota in different stomach microhabitats. We used our previously published 16S rRNA gene sequence data. We retrospectively enrolled a cohort of 132 patients with GC with complete prognostic information and selected 78 normal tissues, 49 peritumoral tissues, and 112 tumoral tissues for microbiota analysis. Patients with different prognoses showed different gastric microbiota compositions and diversity. The association network of the abundant gastric microbiota was more complicated in patients with poor prognoses. In the peritumoral microhabitat of patients with good prognoses, Helicobacter was significantly increased, whereas Halomonas and Shewanella were significantly decreased relative to that in the peritumoral microhabitat of patients with poor prognoses. PiCRUSt analysis revealed that the peritumoral microbiota had more different Kyoto Encyclopedia of Genes and Genomes pathways than did the tumoral and normal microbiota. This study evaluated the long-term prognostic value of the gastric mucosal microbiota in patients with GC. These findings suggested that the characteristic alterations of the gastric mucosal microbiota may be markers for clinical outcomes in these patients.
胃癌（GC）是一种常见的恶性肿瘤，发病率和死亡率都很高。微生物群在人类健康和疾病中起着重要作用。我们旨在研究不同胃微生境中胃微生物群的预后价值。我们使用了之前发表的 16S rRNA 基因序列数据。我们回顾性地纳入了一组 132 例具有完整预后信息的 GC 患者，并选择了 78 个正常组织、49 个癌旁组织和 112 个肿瘤组织进行微生物组分析。具有不同预后的患者表现出不同的胃微生物群组成和多样性。预后不良患者的丰富胃微生物群的关联网络更为复杂。在预后良好的患者的癌旁微生境中，与预后不良的患者相比， Helicobacter 显著增加，而 Halomonas 和 Shewanella 显著减少。PiCRUSt 分析显示，与肿瘤和正常微生物群相比，癌旁微生物群具有更多不同的京都基因与基因组百科全书途径。本研究评估了 GC 患者胃黏膜微生物群的长期预后价值。这些发现表明，胃黏膜微生物群的特征改变可能是这些患者临床结局的标志物。
