D'souza Oshin Jacintha, Gasti Tilak, Hiremani Vishram D, Pinto Jennifer P, Contractor Shafa S, Shettar Arun K, Olivia Diana, Arakera Suresh B, Masti Saraswati P, Chougale Ravindra B
P. G. Department of Studies in chemistry, Karnatak University, Dharwad 580 003, India.
Department of Chemistry, Karnatak Science College, Dharwad 580 003, India.
Int J Biol Macromol. 2023 Jan 15;225:673-686. doi: 10.1016/j.ijbiomac.2022.11.130. Epub 2022 Nov 17.
Natural extract-based bio-composite material for wound healing is gaining much attention due to risk of infection and high cost of commercial wound dressing film causes serious problem on the human well-being. Herein, the study outlines the preparation of Poly (vinyl alcohol)/Chitosan/Basella alba stem extract (BAE) based bio-composite film through solvent casting technique and well characterized for wound healing application. Incorporation of BAE into Poly (vinyl alcohol)/Chitosan matrix has shown existence of secondary interactions confirmed by FT-IR analysis. Good morphology, thermal stability and significant improvement in flexibility (∼63.38 %) of the films were confirmed by SEM, TGA and Mechanical test results, respectively. Hydrophilic property (∼9.04 %), water vapor transmission rate (∼70.07 %), swelling ability (∼14.7 %) and degradation rate (∼14.04 %) were enhanced with increase in BAE content. In-vitro studies have shown good antibacterial activity against foremost infectious bacterial strains S. aureus and E. coli. Additionally, BAE integrated Poly (vinyl alcohol)/Chitosan film has amplified anti-inflammatory (∼79.38 %) property, hemocompatibility and excellent biocompatibility (94.9 %) was displayed by cytotoxicity results. Moreover, in-vitro scratch assay and cell adhesion test results illustrated prominent wound healing (96.5 %) and adhesion. Overall results of the present work proclaim that developed bio-composite film could be utilized as a biomaterial in wound care applications.
基于天然提取物的生物复合材料用于伤口愈合正受到广泛关注，因为感染风险和商业伤口敷料膜的高成本给人类健康带来了严重问题。在此，本研究概述了通过溶剂浇铸技术制备基于聚（乙烯醇）/壳聚糖/落葵茎提取物（BAE）的生物复合膜，并对其在伤口愈合应用方面进行了充分表征。通过傅里叶变换红外光谱（FT-IR）分析证实，将BAE掺入聚（乙烯醇）/壳聚糖基质中存在二次相互作用。扫描电子显微镜（SEM）、热重分析（TGA）和力学测试结果分别证实了该膜具有良好的形态、热稳定性以及柔韧性显著提高（约63.38%）。随着BAE含量的增加，亲水性（约9.04%）、水蒸气透过率（约70.07%）、溶胀能力（约14.7%）和降解率（约14.04%）均有所提高。体外研究表明，该材料对主要感染性细菌菌株金黄色葡萄球菌和大肠杆菌具有良好的抗菌活性。此外，BAE集成的聚（乙烯醇）/壳聚糖膜具有增强的抗炎性能（约79.38%），细胞毒性结果显示其具有血液相容性和优异的生物相容性（94.9%）。此外，并体外划痕试验和细胞粘附试验结果表明其具有显著的伤口愈合能力（96.5%）和粘附性。本工作的总体结果表明，所开发的生物复合膜可作为一种生物材料用于伤口护理应用。
