Shao Chuntian, Zhong Jingwei, Liu Junwen, Yang Yiyan, Li Meilin, Xu Yaqin, Wang Libo
College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, China.
College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, China.
Int J Biol Macromol. 2023 Jan 15;225:484-493. doi: 10.1016/j.ijbiomac.2022.11.105. Epub 2022 Nov 17.
Native polysaccharide was obtained from Lonicera caerulea L. fruits (PLP). Two selenized polysaccharides (PSLP-1 and PSLP-2) were synthesized by the microwave-assisted HNO-NaSeO method, where the selenium (Se) contents were 228 ± 24 and 353 ± 36 μg/g, respectively. The molecular weights of PLP, PSLP-1, and PSLP-2 were 5.9 × 10, 5.6 × 10, and 5.1 × 10 kDa, respectively. PSLP-1 and PSLP-2 contained the same type of monosaccharides as PLP but with different molar ratios. The main chain structure of the native polysaccharide was not changed after selenization. PLP, PSLP-1, and PSLP-2 contained the same six types of glycosidic bonds. Bioactivity assays revealed that the two selenized polysaccharides possessed better antioxidant activities than PLP, but their bile acid-binding abilities and inhibitory activities on acetylcholinesterase (AChE) had weakened. In summary, PLP, PSLP-1, and PSLP-2 may be promising Se supplements in functional foods and inhibitors for the treatment of AChE.
天然多糖是从蓝靛果忍冬果实中获得的（PLP）。通过微波辅助HNO-NaSeO法合成了两种硒化多糖（PSLP-1和PSLP-2），其中硒（Se）含量分别为228±24和353±36μg/g。PLP、PSLP-1和PSLP-2的分子量分别为5.9×10、5.6×10和5.1×10 kDa。PSLP-1和PSLP-2与PLP含有相同类型的单糖，但摩尔比不同。天然多糖硒化后主链结构未发生变化。PLP、PSLP-1和PSLP-2含有相同的六种糖苷键。生物活性测定表明，两种硒化多糖比PLP具有更好的抗氧化活性，但其胆汁酸结合能力和对乙酰胆碱酯酶（AChE）的抑制活性有所减弱。综上所述，PLP、PSLP-1和PSLP-2可能是功能性食品中有前景的硒补充剂以及治疗AChE的抑制剂。
