用于阿尔茨海默病的血液中 microRNA 超分辨率成像。

MicroRNA super-resolution imaging in blood for Alzheimer's disease.

机构信息

Department of Neurosurgery, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul 03722; The Spine and Spinal Cord Institute, Department of Neurosurgery, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul 06273; Biomedical Research Center, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul 06273, Korea.

The Spine and Spinal Cord Institute, Department of Neurosurgery, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul 06273; Biomedical Research Center, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul 06273, Korea.

出版信息

BMB Rep. 2023 Mar;56(2):190-195. doi: 10.5483/BMBRep.2022-0151.

DOI:10.5483/BMBRep.2022-0151

PMID:36404596

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10068345/

Abstract

We propose a novel blood biomarker detection method that uses miRNA super-resolution imaging to enable the early diagnosis of Alzheimer's disease (AD). Here, we report a singlemolecule detection method for visualizing disease-specific miRNA in tissue from an AD mice model, and peripheral blood mononuclear cells (PBMCs) from AD patients. Using optimized Magnified Analysis of Proteome (MAPs), we confirmed that five miRNAs contribute to neurodegenerative disease in the brain hippocampi of 5XFAD and wild-type mice. We also assessed PBMCs isolated from the whole blood of AD patients and a healthy control group, and subsequently analyzed those samples using miRNA super-resolution imaging. We detected more miR-200a-3p expression in the cornu ammonis 1 and dentate gyrus regions of 3 month-old 5XFAD mice than in wild-type mice. Additionally, miRNA super-resolution imaging of blood provides AD diagnosis platform for studying miRNA regulation inside cells at the single molecule level. Our results present a potential liquid biopsy method that could improve the diagnosis of early stage AD and other diseases. [BMB Reports 2023; 56(3): 190-195].

摘要

我们提出了一种新的血液生物标志物检测方法，该方法使用 miRNA 超分辨率成像来实现阿尔茨海默病 (AD) 的早期诊断。在这里，我们报告了一种用于可视化 AD 小鼠模型组织和 AD 患者外周血单核细胞 (PBMC) 中疾病特异性 miRNA 的单分子检测方法。使用优化的放大分析蛋白质组学 (MAPs)，我们证实了在 5XFAD 和野生型小鼠的大脑海马体中，有五种 miRNA 与神经退行性疾病有关。我们还评估了从 AD 患者和健康对照组全血中分离的 PBMC，并随后使用 miRNA 超分辨率成像对这些样本进行了分析。我们发现在 3 个月大的 5XFAD 小鼠的角回和齿状回区域，miR-200a-3p 的表达比野生型小鼠更多。此外，血液的 miRNA 超分辨率成像为研究细胞内 miRNA 调节提供了 AD 诊断平台，达到了单分子水平。我们的研究结果提出了一种潜在的液体活检方法，可提高早期 AD 和其他疾病的诊断能力。[BMB 报告 2023;56(3):190-195]。