Sherafati-Moghadam Mohammad, Pahlavani Hamed Alizadeh, Daryanoosh Farhad, Salesi Mohsen
Department of Sport Sciences, Apadana Institute of Higher Education, Shiraz, Iran.
Department of Physical Education, Farhangian University, Tehran, Iran.
J Diabetes Metab Disord. 2022 Aug 5;21(2):1499-1508. doi: 10.1007/s40200-022-01091-3. eCollection 2022 Dec.
In people with diabetes, one of the problems for patients is muscle wasting and inhibition of the protein synthesis pathway. This study aimed to evaluate the effects of HIIT on protein expression in two skeletal muscles, flexor hallucis longus (FHL) and soleus (SOL) in rats with type 2 diabetes mellitus (T2DM).
Diabetes initially was induced by streptozotocin (STZ) and nicotinamide. Rats with type 2 diabetes were randomly and equally divided into control (n = 6) and HIIT groups (n = 6). After 8 weeks of training, the content of total and phosphorylated proteins of serine/threonine-protein kinases (AKT1), mammalian target of rapamycin (mTOR), P70 ribosomal protein S6 kinase 1 (P70S6K1), and 4E (eIF4E)-binding protein 1 (4E-BP1) in FHL and SOL muscles were measured by Western blotting. While body weight and blood glucose were also controlled.
In the HIIT training group, compared to the control group, a significant increase in the content of AKT1 (0.003) and mTOR (0.001) proteins was observed in the FHL muscle. Also, after 8 weeks of HIIT training, protein 4E-BP1 (0.001) was increased in SOL muscle. However, there was no significant change in other proteins in FHL and SOL muscle.
In rats with type 2 diabetes appear to HIIT leading to more protein expression of fast-twitch muscles than slow-twitch muscles. thus likely HIIT exercises can be an important approach to increase protein synthesis and prevent muscle atrophy in people with type 2 diabetes.
在糖尿病患者中，患者面临的问题之一是肌肉萎缩和蛋白质合成途径受到抑制。本研究旨在评估高强度间歇训练（HIIT）对2型糖尿病（T2DM）大鼠两块骨骼肌——拇长屈肌（FHL）和比目鱼肌（SOL）中蛋白质表达的影响。
最初通过链脲佐菌素（STZ）和烟酰胺诱导糖尿病。将2型糖尿病大鼠随机且等分为对照组（n = 6）和HIIT组（n = 6）。训练8周后，通过蛋白质免疫印迹法测量FHL和SOL肌肉中丝氨酸/苏氨酸蛋白激酶（AKT1）、雷帕霉素靶蛋白（mTOR）、P70核糖体蛋白S6激酶1（P70S6K1）和4E（eIF4E）结合蛋白1（4E - BP1）的总蛋白和磷酸化蛋白含量。同时也对体重和血糖进行了控制。
在HIIT训练组中，与对照组相比，FHL肌肉中AKT1（0.003）和mTOR（0.001）蛋白含量显著增加。此外，HIIT训练8周后，SOL肌肉中4E - BP1蛋白（0.001）增加。然而，FHL和SOL肌肉中的其他蛋白没有显著变化。
在2型糖尿病大鼠中，HIIT似乎导致快肌纤维比慢肌纤维有更多的蛋白质表达。因此，HIIT运动可能是增加2型糖尿病患者蛋白质合成和预防肌肉萎缩的重要方法。
