Geng Nan, Sun Guojin, Liu Wen-Jia, Gao Bin-Cheng, Sun Cong, Xu Cundong, Hua Ertian, Xu Lin
Key Laboratory for Technology in Rural Water Management of Zhejiang Province, Zhejiang University of Water Resources and Electric Power, Hangzhou, People's Republic of China.
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, People's Republic of China.
Evol Bioinform Online. 2022 Nov 15;18:11769343221134400. doi: 10.1177/11769343221134400. eCollection 2022.
is an emergent marine pathogen and is the cause of a deadly septicemia. However, the evolution mechanism of antibiotic-resistant genes (ARGs) is still unclear. Twenty-two high-quality complete genomes of were obtained and grouped into 16 clinical isolates and 6 environmental isolates. Genomic annotations found 23 ARG orthologous genes, among which 14 ARGs were shared by and other members. Furthermore, those ARGs were located in their chromosomes, rather than in the plasmids. Phylogenomic reconstruction based on single-copy orthologous protein sequences and ARG protein sequences revealed that clinical and environmental isolates were in a scattered distribution. The calculation of non-synonymous and synonymous substitutions indicated that most of ARGs evolved under purifying selection with the / ratios lower than one, while , and in several clinical isolates evolved under the positive selection with / ratios >1. Our result indicated that antibiotic-resistant armory was not only confined to clinical isolates, but to environmental ones as well and clinical isolates inclined to accumulate beneficial non-synonymous substitutions that could be retained to improve competitiveness.
是一种新兴的海洋病原体，是致命败血症的病因。然而，抗生素抗性基因（ARGs）的进化机制仍不清楚。获得了22个高质量的完整基因组，并将其分为16个临床分离株和6个环境分离株。基因组注释发现了23个ARG直系同源基因，其中14个ARG由和其他成员共享。此外，这些ARG位于它们的染色体上，而不是质粒中。基于单拷贝直系同源蛋白序列和ARG蛋白序列的系统发育重建表明，临床和环境分离株呈分散分布。非同义替换和同义替换的计算表明，大多数ARG在纯化选择下进化，/比值低于1，而在几个临床分离株中，和在正选择下进化，/比值>1。我们的结果表明，抗生素抗性库不仅限于临床分离株，也包括环境分离株，并且临床分离株倾向于积累有益的非同义替换，这些替换可以保留以提高竞争力。
