Marjani Somayeh, Nezhadali Masoumeh, Hekmat Azadeh, Yeganeh Marjan Zarif
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran.
Iran J Public Health. 2022 May;51(5):1143-1151. doi: 10.18502/ijph.v51i5.9429.
Visfatin is known as one of the adipokines associated with the development of inflammation, but its role in the pathogenesis of nonalcoholic fatty liver is less known so far. We aimed to investigate the association between polymorphism and insulin resistance and non-alcoholic fatty liver disease (NAFLD).
This case-control study was performed on 80 patients with NAFLD as well as 80 healthy participants as controls referred to Amir Al-Momenin and Bouali hospitals in Tehran. Genotyping was performed using PCR-RFLP method. Plasma concentrations of visfatin and insulin were measured using ELISA kit. The fasting blood glucose, TC, TG, LDL-C, HDL-C, ALT, AST, SBP, DBP, and BMI levels were measured using the standard methods. Statistical analysis was also performed using SPSS software.
A significant difference was found in Visfatin level in the patients with NAFLD compared to this level in healthy individuals. The levels of HDL-C and LDL-C in healthy individuals and triglyceride in patients for GG, AG, and AA genotypes carriers also were significantly different. There was a significant relationship between polymorphism and insulin resistance; however, no association was found between this polymorphism and NAFLD. Notably, Visfatin showed a significant association with age (all individuals), body mass index (healthy individuals), insulin, and HOMA (in patients).
Visfatin levels reduced in patients with NAFLD. Moreover, rs4730153 polymorphism was indicated to be associated with both lipid metabolism and insulin resistance, but no association was found between this polymorphism and nonalcoholic fatty liver disease.
内脂素是已知的与炎症发展相关的脂肪因子之一，但迄今为止其在非酒精性脂肪肝发病机制中的作用尚鲜为人知。我们旨在研究基因多态性与胰岛素抵抗及非酒精性脂肪性肝病（NAFLD）之间的关联。
本病例对照研究对转诊至德黑兰阿米尔·穆民医院和布阿利医院的80例NAFLD患者以及80名健康参与者进行。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法进行基因分型。使用酶联免疫吸附测定（ELISA）试剂盒测定血浆内脂素和胰岛素浓度。采用标准方法测量空腹血糖、总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）、谷丙转氨酶（ALT）、谷草转氨酶（AST）、收缩压（SBP）、舒张压（DBP）和体重指数（BMI）水平。还使用SPSS软件进行统计分析。
与健康个体相比，NAFLD患者的内脂素水平存在显著差异。GG、AG和AA基因型携带者的健康个体中的HDL-C和LDL-C水平以及患者中的甘油三酯水平也存在显著差异。基因多态性与胰岛素抵抗之间存在显著关系；然而，未发现该多态性与NAFLD之间存在关联。值得注意的是，内脂素与年龄（所有个体）、体重指数（健康个体）、胰岛素和稳态模型评估胰岛素抵抗指数（HOMA）（患者）之间存在显著关联。
NAFLD患者的内脂素水平降低。此外，rs⁴⁷³⁰¹⁵³多态性被表明与脂质代谢和胰岛素抵抗均相关，但未发现该多态性与非酒精性脂肪性肝病之间存在关联。
