Yang Yu, Lai Qingzhong, Wang Chenyu, Zhou Gaoli
Medical Laboratory, Fuyang District Maternal and Child Health Hospital, Hangzhou 311400, China.
Department of Chinese Medical Massage, Zhejiang Integrated Traditional and Western Medicine Hospital, Hangzhou 310003, China.
Evid Based Complement Alternat Med. 2022 Nov 11;2022:7609785. doi: 10.1155/2022/7609785. eCollection 2022.
is the well-knownfood-medicine herb with the special taste and smell. It is also widely used in south China for prevention of various chronic pulmonary inflammatory diseases including asthma. However, the active ingredients and therapeutic mechanism of this herb remain obscure. In this study, network pharmacology technology was employed to investigate the effects of aqueous extract (HHAE) on OVA-induced airway hyperresponsiveness and inflammation. The results showed that six compounds (isoramanone, kaempferol, 1-methyl-2-nonacosyl-4-quinolone, C09747, spinasterol, and quercetin) were found to be mainly responsible for the therapeutic effects of the herb, which totally regulated the expressions of 168 asthma-related proteins. All those targets involved in the signal transduction of the prolactin signaling pathway, central carbon metabolism in cancer, EGFR tyrosine kinase inhibitor resistance, endocrine resistance, and VEGF signaling pathway. The in vivo experiment also revealed that orally administrated with HHAE alleviated airway hyperresponsiveness and inflammation in OVA-induced asthmatic mice. It significantly decreased the counts of neutrophils, eosinophils, and lymphocytes as well as the levels of IL-1, IL-4, IL-6, and IL-13 in BALF of asthmatic mice. Mechanically, HHAE downregulated both the mRNA and protein expressions of p38 MAPK, PI3K, AKT, and VEGF in the lung tissues of asthmatic mice. Therefore, HHAE improved OVA-induced airway hyperresponsiveness and inflammation in mice and could be a potential supplement for asthma treatment.
是一种具有独特味道和气味的著名食药两用草药。它在中国南方也被广泛用于预防包括哮喘在内的各种慢性肺部炎症性疾病。然而，这种草药的活性成分和治疗机制仍不清楚。在本研究中，采用网络药理学技术研究水提取物（HHAE）对卵清蛋白（OVA）诱导的气道高反应性和炎症的影响。结果表明，六种化合物（异紫罗酮、山奈酚、1-甲基-2-二十九烷基-4-喹诺酮、C09747、菠菜甾醇和槲皮素）被发现是该草药治疗作用的主要原因，它们共同调节了168种哮喘相关蛋白的表达。所有这些靶点都参与了催乳素信号通路、癌症中的中心碳代谢、表皮生长因子受体（EGFR）酪氨酸激酶抑制剂耐药性、内分泌耐药性和血管内皮生长因子（VEGF）信号通路的信号转导。体内实验还表明，口服HHAE可减轻OVA诱导的哮喘小鼠的气道高反应性和炎症。它显著降低了哮喘小鼠支气管肺泡灌洗液（BALF）中中性粒细胞、嗜酸性粒细胞和淋巴细胞的数量以及白细胞介素-1（IL-1）、白细胞介素-4（IL-4）、白细胞介素-6（IL-6）和白细胞介素-13（IL-13）的水平。机制上，HHAE下调了哮喘小鼠肺组织中p38丝裂原活化蛋白激酶（p38 MAPK）、磷脂酰肌醇-3激酶（PI3K）、蛋白激酶B（AKT）和VEGF的mRNA和蛋白表达。因此，HHAE改善了OVA诱导的小鼠气道高反应性和炎症，可能是哮喘治疗的一种潜在补充剂。
