Bioorganic Analytics, Institute for Inorganic and Analytical Chemistry, Friedrich Schiller University, Lessingstrasse 8, D-07743 Jena, Germany.
MPG Fellow Group, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, D-07745 Jena, Germany.
Mar Drugs. 2022 Nov 19;20(11):727. doi: 10.3390/md20110727.
Osmolytes are naturally occurring organic compounds that protect cells against various forms of stress. Highly polar, zwitterionic osmolytes are often used by marine algae and bacteria to counteract salinity or temperature stress. We investigated the effect of several stress conditions including different salinities, temperatures, and exposure to organic metabolites released by the alga on the halophilic heterotrophic bacterium . Using ultra-high-performance liquid chromatography (UHPLC) on a ZIC-HILIC column and high-resolution electrospray ionization mass spectrometry, we simultaneously detected and quantified the eleven highly polar compounds dimethylsulfoxonium propionate (DMSOP), dimethylsulfoniopropionate (DMSP), gonyol, cysteinolic acid, ectoine, glycine betaine (GBT), carnitine, sarcosine, choline, proline, and 4-hydroxyproline. All compounds are newly described in and potentially involved in physiological functions essential for bacterial survival under variable environmental conditions. We report that adaptation to various forms of stress is accomplished by adjusting the pattern and amount of the zwitterionic metabolites.
渗透物是天然存在的有机化合物，可保护细胞免受各种形式的压力。高度极性、两性离子的渗透物常被海洋藻类和细菌用于抵消盐度或温度压力。我们研究了几种应激条件的影响，包括不同的盐度、温度以及暴露于藻类释放的有机代谢物对嗜盐异养菌的影响。使用 ZIC-HILIC 柱的超高效液相色谱 (UHPLC) 和高分辨率电喷雾电离质谱，我们同时检测和定量了 11 种高极性化合物二甲基亚砜丙酸盐 (DMSOP)、二甲基亚磺丙酸盐 (DMSP)、岩藻黄素、半胱氨酸酸、章鱼胺、甘氨酸甜菜碱 (GBT)、肉碱、肌氨酸、胆碱、脯氨酸和 4-羟基脯氨酸。所有化合物均为首次在 中描述，可能与细菌在可变环境条件下生存的生理功能有关。我们报告说，通过调整两性离子代谢物的模式和数量来实现对各种形式的压力的适应。
