Department of Radiobiology, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, University Science Park for Biomedicine, 845 05 Bratislava, Slovakia.
Department of Laboratory Medicine, Faculty of Health Care, Catholic University in Ružomberok, 034 01 Ružomberok, Slovakia.
Int J Mol Sci. 2022 Nov 19;23(22):14391. doi: 10.3390/ijms232214391.
In a previous study of the effects of intermittent extremely low frequency (ELF) magnetic fields (MF) on umbilical cord blood lymphocytes (UCBL), we evaluated MF amplitudes between 6 µT and 24 µT and found an effect only for those below 13 µT. This suggested the existence of an amplitude window. In this brief communication, we further tested this hypothesis. UCBLs from healthy newborns were isolated and exposed for 72 h to an intermittent ELF-MF (triangular, 7.8 Hz, 250 s ON/250 s OFF) with 6 different amplitudes between 3 µT and 12 µT, utilizing an oblong coil. Percentage of viable, early apoptotic (EA), and late apoptotic/necrotic (LAN) cells were determined by flow cytometry. Moreover, reactive oxygen species (ROS) were determined at 1 h and 3 h of the exposure. Like in our previous work, neither EA, nor LAN, nor ROS were statistically significantly affected by the intermittent ELF-MF. However, the percentage of viable cells was decreased by exposure to the fields with intensities of 6.5 µT and 12 µT (p < 0.05; and p = 0.057 for 8.5 µT). ELF-MF decreased the percentage of viable cells for fields down to 6.5 µT, but not for 5 µT, 4 µT, or 3 µT. Combined with our previous findings, the results reported here indicate an amplitude window effect between 6 µT and 13 µT. The obtained data are in line with a notion of amplitude and frequency windows, which request scanning of both amplitude and frequency while studying the ELF-MF effects.
在之前关于间歇性极低频(ELF)磁场(MF)对脐带血淋巴细胞(UCBL)影响的研究中,我们评估了 6µT 至 24µT 之间的 MF 幅度,仅发现低于 13µT 的幅度有影响。这表明存在幅度窗口。在本简要通讯中,我们进一步测试了这一假设。从健康新生儿中分离出 UCBL,并将其暴露于间歇性 ELF-MF(三角波,7.8Hz,250s ON/250s OFF)中 72 小时,MF 幅度在 3µT 至 12µT 之间有 6 个不同值,利用一个长方形线圈。通过流式细胞术测定活细胞、早期凋亡(EA)和晚期凋亡/坏死(LAN)细胞的百分比。此外,在暴露 1 小时和 3 小时时测定活性氧物质(ROS)。与我们之前的工作一样,EA、LAN 或 ROS 均不受间歇性 ELF-MF 的显著影响。然而,暴露于 6.5µT 和 12µT 的场中,活细胞的百分比降低(p<0.05;8.5µT 时 p=0.057)。ELF-MF 降低了场中活细胞的百分比,低至 6.5µT,但 5µT、4µT 或 3µT 则不然。结合我们之前的发现,这里报告的结果表明 6µT 至 13µT 之间存在幅度窗口效应。获得的数据符合幅度和频率窗口的概念,即在研究 ELF-MF 效应时需要同时扫描幅度和频率。
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