Antimikrop Ar-Ge ve Biyosidal Analiz Merkezi, Nasuh Akar Mah. Süleyman Hacıabdullahoğlu Cad. No: 37/1, Çankaya, Ankara, Turkey.
Antimikrop Ar-Ge ve Biyosidal Analiz Merkezi, Nasuh Akar Mah. Süleyman Hacıabdullahoğlu Cad. No: 37/1, Çankaya, Ankara, Turkey.
J Virol Methods. 2023 Feb;312:114660. doi: 10.1016/j.jviromet.2022.114660. Epub 2022 Nov 24.
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused a global pandemic mainly through air transmission. Novel variants are emerging and thus innovative methods of air disinfection still warrant exploration. Essential oils with virucidal properties may be an alternative to known air disinfectants.
Analysis of virucidal potential of thyme oil vapor phase against airborne SARS-CoV-2.
Chemical composition of thyme oil was analyzed by Gas chromatography-mass spectrometry.Thyme oil was tested in solution in different dilutions against soluble SARS-CoV-2. For air disinfection analysis, different volumes of thyme oil were placed in a container with hot water and its vapor phase was tested against airborne/aerosolized SARS-CoV-2 in a 30 m room. The aerosolized virus was collected in a gelatine filter using a vacuum system after the test and the collected virus was quantified utilizing inoculations of serial dilutions into 96-well plates with VERO E6 cells.
The main component of thyme oil was carvacrol. Thyme oil had virucidal action both in solution and in the air. Thyme oil at 1/1000 dilution (volume/volume, final concentration) in a solution eliminated more than 99,99% of SARS-CoV-2 in the solution in 60 min. In air disinfection tests in 30 m room, vapor phase of 40 ml of extracted thyme oil eliminated more than 99,99% (>4 LOG) of airborne SARS-CoV-2, and vapor phase of 20 ml of thyme oil resulted in elimination of 90,88% (1,04 LOG) airborne SARS-CoV-2 in 60 min.
We have shown that vapor phase of thyme oil inactivates more than 99,99% of airborne SARS-CoV-2 in a room for the first time to the best of our knowledge. This finding may have implications on the use of thyme oil as a potential air disinfectant against SARS-CoV-2.
严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）主要通过空气传播引发了全球大流行。新型变体不断出现，因此仍需要探索创新的空气消毒方法。具有抗病毒特性的精油可能是已知空气消毒剂的替代品。
分析 thyme oil 蒸汽相针对空气中 SARS-CoV-2 的抗病毒潜力。
通过气相色谱-质谱联用分析 thyme oil 的化学成分。在不同稀释度下，thyme oil 溶液测试针对可溶性 SARS-CoV-2 的抗病毒作用。对于空气消毒分析，将不同体积的 thyme oil 放置在盛有热水的容器中，测试其蒸汽相针对 30m 房间内空气中/气溶胶 SARS-CoV-2 的抗病毒作用。测试后，使用真空系统将气溶胶化病毒收集到明胶过滤器中，并利用将连续稀释液接种到 96 孔板中的方法对收集到的病毒进行定量，该稀释液接种到了 VERO E6 细胞中。
thyme oil 的主要成分是香芹酚。thyme oil 在溶液中和空气中均具有抗病毒作用。thyme oil 在溶液中的 1/1000 稀释度（体积/体积，终浓度）下，在 60 分钟内消除了超过 99.99%的 SARS-CoV-2。在 30m 房间的空气消毒测试中，40ml 提取的 thyme oil 蒸汽相消除了超过 99.99%（>4 LOG）的空气传播 SARS-CoV-2，而 20ml 的 thyme oil 蒸汽相在 60 分钟内消除了 90.88%（1.04 LOG）的空气传播 SARS-CoV-2。
我们首次证明了 thyme oil 蒸汽相在房间内可以使超过 99.99%的空气传播 SARS-CoV-2 失活，据我们所知，这一发现可能对 thyme oil 作为 SARS-CoV-2 的潜在空气消毒剂的使用具有重要意义。
