Salehi-Arjmand Hossein, Akhoundi Maryam, Akramian Morteza, Khadivi Ali, Tunç Yazgan
Department of Medicinal and Aromatic Plants, Faculty of Agriculture and Natural Resources, Arak University, Arak, 38156-8-8349, Iran.
Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, Arak University, Arak, 38156-8-8349, Iran.
Sci Rep. 2025 May 9;15(1):16272. doi: 10.1038/s41598-025-00768-y.
The present study aimed to investigate the physiochemical responses of Thymus vulgaris L. to salinity stress and was arranged in a two-factorial experiment based on a completely randomized design (CRD) with three replications under floating hydroponic conditions. The effect of salinity stress at three levels of 0, 100, and 200 mM NaCl for seven different time courses of 0, 6, 12, 24, 48, 72, 96, and 120 h on 3 months garden thyme plant (4 months after seed cultivation and 3 months after transplanting in hydroponic system) was investigated. The results showed that different concentrations of NaCl and different times of salinity stress had no significant effect on the fresh mass, while photosynthetic pigment content and DPPH (2,2-Diphenyl-1-picrylhydrazyl) radical scavenging activity were significantly decreased under high concentrations of sodium chloride and 72-120 h after salt stresses. In addition, the increase of salinity stress (100 and 200 mM) in 72-120 h treatment significantly increased compatible osmolytes, such as proline content. On the other hand, the activity of non-enzymatic antioxidants, such as phenolics and flavonoids under salt stress treatment with concentrations of 100 and 200 mM sodium chloride in the floating hydroponic system during 72-120 h showed significant increases. In general, it can be concluded that high concentrations of sodium chloride in floating hydroponic systems can cause significant adverse effects on the physical and biochemical properties of the garden thyme plant.
本研究旨在探究百里香对盐胁迫的生理生化响应，并在漂浮水培条件下，基于完全随机设计（CRD）进行双因素实验，设置三个重复。研究了0、100和200 mM NaCl三个盐胁迫水平，以及0、6、12、24、48、72、96和120 h七个不同时间进程，对3个月大的花园百里香植株（种子种植后4个月，水培系统移栽后3个月）的影响。结果表明，不同浓度的NaCl和不同时长的盐胁迫对鲜重无显著影响，而在高浓度氯化钠处理及盐胁迫72 - 120 h后，光合色素含量和DPPH（2,2 - 二苯基 - 1 - 苦基肼）自由基清除活性显著降低。此外，在72 - 120 h处理中，盐胁迫增加（100和200 mM）显著增加了脯氨酸等相容性渗透物质的含量。另一方面，在72 - 120 h期间，漂浮水培系统中100和200 mM氯化钠浓度的盐胁迫处理下，酚类和黄酮类等非酶抗氧化剂的活性显著增加。总体而言，可以得出结论，漂浮水培系统中的高浓度氯化钠会对花园百里香植株的物理和生化特性产生显著不利影响。
