Podlesnaya P A, Kovaleva O V, Petrenko A A, Gratchev A N
N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2022 Nov;174(1):147-151. doi: 10.1007/s10517-022-05664-3. Epub 2022 Nov 28.
An experimental cell-based model was developed to study antibody-independent cytotoxic activity of macrophages that allows selection of derivative tumor cells resistant to this activity and clarification of the mechanisms responsible for this selection. Cytotoxic macrophages showing antibody-independent cytotoxic activity were obtained. Derivatives of PC3 and H1299 cells resistant to cytotoxic macrophage activity were generated. The main characteristics of the obtained derivative cells were studied. The proposed experimental model can be used as a tool for studying the mechanisms of the development of tumor cells resistance to antibody-independent cytotoxic activity of macrophages.
建立了一种基于细胞的实验模型，以研究巨噬细胞的非抗体依赖性细胞毒性活性，该模型允许选择对这种活性具有抗性的衍生肿瘤细胞，并阐明负责这种选择的机制。获得了具有非抗体依赖性细胞毒性活性的细胞毒性巨噬细胞。产生了对细胞毒性巨噬细胞活性具有抗性的PC3和H1299细胞衍生物。研究了所获得的衍生细胞的主要特征。所提出的实验模型可作为研究肿瘤细胞对巨噬细胞非抗体依赖性细胞毒性活性产生抗性机制的工具。
