植物提取物介导的银纳米粒子（AgNPs）的合成及其生物学活性。
Phyto-Extract-Mediated Synthesis of Silver Nanoparticles (AgNPs) and Their Biological Activities.
机构信息
Department of Botany, Kohat University of Science and Technology, Kohat 26000, Pakistan.
Department of Botany, Abdul Wali Khan University Mardan, Pakistan.
出版信息
Biomed Res Int. 2022 Nov 16;2022:9845022. doi: 10.1155/2022/9845022. eCollection 2022.
BACKGROUND
Nanotechnology finds broad applications in the field of nanomedicine, an emerging new field used for diagnosis, treatment, prevention of diseases, and improvement of health.
OBJECTIVES
To synthesize silver nanoparticles (AgNPs) from and and to carry out their antimicrobial, insecticidal, and phytotoxic activities, a step toward the new range of nanomedicines.
METHODS
Silver nanoparticles were synthesized from and by chemical reduction method, and further biological activities of these nanoparticles were compared with crude methanolic extract, prepared through cold maceration process, at the concentration of 50 mg/ml.
RESULTS
Among all tested bacterial pathogens, crude extract of showed a statistically high significant inhibition zone in millimeter against (21; < 0.01). AgNPs showed highly significant result against (14; < 0.01). In comparison with crude extracts, AgNPs showed statistically significant ( < 0.01) results against (AgNPs, 14; crude, 8.33 mm). Crude extract showed significant inhibition zone against two bacterial strains, (crude, 21; AgNPs, 11.67 mm) and (crude, 11.33; AgNPs, 8 mm). Crude extracts of showed the significant activity against ( < 0.01; 11.33 mm). Silver nanoparticles of exhibited the highest significant activity against and while AgNPs of were active only against . Extracts of and showed increasing phytotoxic activity with increasing concentrations. The highest significant inhibition was obtained for crude extract (46.7) and AgNPs (45.7) of at 1000 g/ml. Insecticidal activity of crude and AgNPs of both plants showed significant inhibition against all tested insects with increasing time intervals, and the highest significant result was obtained at 72 hours with a value of < 0.01 except .
CONCLUSIONS
Both crude and AgNPs showed potent activity; however, in comparison, silver nanoparticles showed slightly enhanced activity. Crude and AgNPs of both plants showed good phytotoxic and insecticidal inhibition. Antimicrobial studies of AgNPs on diseases causing pathogens open a door for new antimicrobial agents and could be the answer to antibiotic resistance after further analysis.
背景
纳米技术在纳米医学领域有广泛的应用，这是一个新兴的领域，用于疾病的诊断、治疗、预防和健康改善。
目的
从 和 中合成银纳米粒子（AgNPs），并对其进行抗菌、杀虫和植物毒性活性的研究，为新型纳米药物的开发奠定基础。
方法
通过化学还原法从 和 中合成银纳米粒子，并将其与通过冷浸提过程制备的浓度为 50mg/ml 的甲醇粗提物进行比较。
结果
在所测试的所有细菌病原体中， 的粗提物在毫米（mm）水平上对 表现出统计学上的高度显著抑制作用（21； < 0.01）。AgNPs 对 表现出高度显著的结果（14； < 0.01）。与粗提取物相比，AgNPs 对 的抑制作用具有统计学意义（ < 0.01）（AgNPs，14；粗提物，8.33mm）。粗提物对两种细菌菌株 和 表现出显著的抑制作用（粗提物，21；AgNPs，11.67mm）和 （粗提物，11.33；AgNPs，8mm）。 的粗提物对 表现出显著的活性（ < 0.01；11.33mm）。 和 的 AgNPs 对 和 表现出最高的显著活性，而 的 AgNPs 仅对 有效。 和 的提取物的植物毒性活性随着浓度的增加而增加。在 1000μg/ml 时， 的粗提物（46.7）和 AgNPs（45.7）获得了最高的显著抑制作用。两种植物的粗提物和 AgNPs 的杀虫活性均表现出随着时间间隔的增加对所有测试昆虫的显著抑制作用，在 72 小时时获得了最高的显著结果（ < 0.01），除 外。
结论
粗提物和 AgNPs 均表现出较强的活性；然而，相比之下，AgNPs 表现出略微增强的活性。两种植物的粗提物和 AgNPs 均表现出良好的植物毒性和杀虫抑制作用。AgNPs 对致病病原体的抗菌研究为新型抗菌剂打开了大门，并可能为进一步分析后抗生素耐药性提供答案。
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