School of Life Sciences, North Maharashtra University, Post Box-80, Jalgaon, 425001, Maharashtra, India.
Parasitol Res. 2012 May;110(5):1815-22. doi: 10.1007/s00436-011-2704-x. Epub 2011 Nov 18.
In the present study activity of silver nanoparticles (AgNPs) synthesized using Plumeria rubra plant latex against second and fourth larval instar of Aedes aegypti and Anopheles stephensi was determined. Range of concentrations of synthesized AgNps (10, 5, 2.5, 1.25, 0.625, 0.3125 ppm) and aqueous crude latex (1,000, 500, 250, 125, 62.50, 31.25 ppm) were tested against larvae of A. aegypti and A. Stephensi. The synthesized AgNps from P. rubra latex were highly toxic than crude latex extract in both mosquito species. The LC(50) values for second and fourth larval instars after 24 h of crude latex exposure were 1.49, 1.82 ppm against A. aegypti and 1.10, 1.74 ppm against A. stephensi respectively. These figures were 181.67, 287.49 ppm against A. aegypti and 143.69, 170.58 ppm against A. stephensi respectively for crude latex extract. The mortality rates were positively correlated with the concentration of AgNPs. The characterization studies of synthesized AgNPs by UV-Vis spectrophotometry, transmission electron microscopy (TEM), Particle size analysis (PSA) and zeta potential confirmed the spherical shape and size (32-200 nm) of silver nanoparticles along with stability. Toxicity studies carried out against non-target fish species Poecilia reticulata, the most common organism in the habitats of A. aegypti and A. stephensi showed no toxicity at LC(50) and LC(90) doses of the AgNPs. This is the first report on mosquito larvicidal activity of latex synthesized nanoparticles.
在本研究中，测定了用鸡蛋花植物乳液合成的银纳米粒子（AgNPs）对埃及伊蚊和致倦库蚊第二和第四龄幼虫的活性。测试了合成的 AgNPs（10、5、2.5、1.25、0.625、0.3125ppm）和水提粗乳液（1000、500、250、125、62.50、31.25ppm）对埃及伊蚊和致倦库蚊幼虫的作用。鸡蛋花乳液合成的 AgNPs 比两种蚊种的粗乳液提取物毒性更高。粗乳液暴露 24 小时后，第二和第四龄幼虫的 LC50 值分别为 1.49、1.82ppm 对埃及伊蚊和 1.10、1.74ppm 对致倦库蚊。而粗乳液提取物的相应值分别为 181.67、287.49ppm 对埃及伊蚊和 143.69、170.58ppm 对致倦库蚊。死亡率与 AgNPs 的浓度呈正相关。通过紫外-可见分光光度法、透射电子显微镜（TEM）、粒度分析（PSA）和zeta 电位对合成的 AgNPs 进行的表征研究证实了银纳米粒子的球形形状和尺寸（32-200nm）以及稳定性。对非靶标鱼类物种孔雀鱼进行的毒性研究表明，在 AgNPs 的 LC50 和 LC90 剂量下，没有表现出毒性。这是关于乳胶合成纳米粒子对蚊子幼虫的杀幼虫活性的首次报道。
