Vivek Marimuthu, Kumar Palanisamy Senthil, Steffi Sesurajan, Sudha Sellappa
Department of Biotechnology, School of Life Sciences, Karpagam University, Coimbatore, Tamil Nadu, India.
Avicenna J Med Biotechnol. 2011 Jul;3(3):143-8.
The synthesis, characterization and application of biologically synthesized nanomaterials are an important aspect in nanotechnology. The present study deals with the synthesis of silver nanoparticles (Ag-NPs) using the aqueous extract of red seaweed Gelidiella acerosa as the reducing agent to study the antifungal activity. The formation of Ag-NPs was confirmed by UV-Visible Spectroscopy, X-Ray Diffraction (XRD) pattern, Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). The synthesized Ag-NPs was predominately spherical in shape and polydispersed. Fourier Transform Infra-Red (FT-IR) spectroscopy analysis showed that the synthesized nano-Ag was capped with bimolecular compounds which are responsible for reduction of silver ions. The antifungal effects of these nanoparticles were studied against Humicola insolens (MTCC 4520), Fusarium dimerum (MTCC 6583), Mucor indicus (MTCC 3318) and Trichoderma reesei (MTCC 3929). The present study indicates that Ag-NPs have considerable antifungal activity in comparison with standard antifungal drug, and hence further investigation for clinical applications is necessary.
生物合成纳米材料的合成、表征及应用是纳米技术的一个重要方面。本研究利用红藻鹿角沙菜的水提取物作为还原剂来合成银纳米颗粒（Ag-NPs），以研究其抗真菌活性。通过紫外可见光谱、X射线衍射（XRD）图谱、扫描电子显微镜（SEM）和透射电子显微镜（TEM）证实了Ag-NPs的形成。合成的Ag-NPs主要呈球形且多分散。傅里叶变换红外（FT-IR）光谱分析表明，合成的纳米银被双分子化合物包覆，这些化合物负责银离子的还原。研究了这些纳米颗粒对特异腐质霉（MTCC 4520）、双隔镰孢（MTCC 6583）、印度毛霉（MTCC 3318）和里氏木霉（MTCC 3929）的抗真菌效果。本研究表明，与标准抗真菌药物相比，Ag-NPs具有相当的抗真菌活性，因此有必要进一步研究其临床应用。
