School of Life Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
Faculty of Engineering, University of Nottingham, Nottingham, NG7 2RD, UK.
Adv Mater. 2024 Oct;36(43):e2208364. doi: 10.1002/adma.202208364. Epub 2022 Dec 18.
Wound healing is a complex biological process involving close crosstalk between various cell types. Dysregulation in any of these processes, such as in diabetic wounds, results in chronic nonhealing wounds. Fibroblasts are a critical cell type involved in the formation of granulation tissue, essential for effective wound healing. 315 different polymer surfaces are screened to identify candidates which actively drive fibroblasts toward either pro- or antiproliferative functional phenotypes. Fibroblast-instructive chemistries are identified, which are synthesized into surfactants to fabricate easy to administer microparticles for direct application to diabetic wounds. The pro-proliferative microfluidic derived particles are able to successfully promote neovascularization, granulation tissue formation, and wound closure after a single application to the wound bed. These active novel bio-instructive microparticles show great potential as a route to reducing the burden of chronic wounds.
伤口愈合是一个复杂的生物学过程，涉及各种细胞类型之间的密切相互作用。这些过程中的任何一个失调，如糖尿病伤口，都会导致慢性不愈合的伤口。成纤维细胞是参与肉芽组织形成的关键细胞类型，对于有效的伤口愈合至关重要。我们筛选了 315 种不同的聚合物表面，以确定能够积极促使成纤维细胞向促有丝分裂或抗有丝分裂功能表型发展的候选物。我们确定了成纤维细胞指令性化学物质，并将其合成到表面活性剂中，以制造易于管理的微颗粒，直接应用于糖尿病伤口。经微流控处理后具有促有丝分裂作用的颗粒，在单次应用于伤口床后，能够成功促进血管新生、肉芽组织形成和伤口闭合。这些新型的活性生物指令性微颗粒具有很大的潜力，可以减轻慢性伤口的负担。
