Schlake W, Meyer E M, Grundmann E
Pathol Res Pract. 1978 Oct;163(2):173-82. doi: 10.1016/s0344-0338(78)80087-9.
A strong activity of acid alpha-naphthyl acetate esterase (ANAE) in T lymphocytes and macrophages within mouse lymphoid organs is demonstrable even after paraffin-embedding, if a rapid embedding procedure with acetone as dehydrating agent is used. The lymphoid tissue structure is better preserved in paraffin sections procured after this rapid embedding procedure than in frozen sections commonly used for histochemical demonstration of ANAE. In the paraffin sections the pattern of the ANAE reaction allows a clear delineation of the T and B areas in the lymphoid organs, and the intracellular location of the focal ANAE activity in T lymphocytes is readily discernible.
即使经过石蜡包埋，如果采用以丙酮作为脱水剂的快速包埋程序，小鼠淋巴器官内的T淋巴细胞和巨噬细胞中酸性α-萘乙酸酯酶（ANAE）仍具有较强活性。采用这种快速包埋程序制备的石蜡切片中，淋巴组织结构比常用于ANAE组织化学显示的冰冻切片保存得更好。在石蜡切片中，ANAE反应模式能够清晰勾勒出淋巴器官中的T区和B区，并且T淋巴细胞中局灶性ANAE活性的细胞内定位易于辨别。
