Department of General Surgery and Surgical-Medical Specialties, School of Dentistry, University of Catania, Catania, Italy.
Adv Exp Med Biol. 2023;1396:301-313. doi: 10.1007/978-981-19-5642-3_19.
Genomic editing technology has been developed since 2010 through the use of some techniques, such as the clustered regularly interspaced short palindromic repeat (CRISPR) DNA sequences/CRISPR-associated (Cas) type-9 method, or through genetic manipulation tools derived from host response systems from some microbes (e.g., bacteria) against plasmids and viruses. The introduction of the CRISPR/Cas9 method as a genome-editing instrument represented an important step in the advancement of the genome-editing method thanks to the ease and effectiveness of use as well as the great adaptability to different biomedical areas. This paragraph will discuss all conceived technologies and new perspectives that can be applied in treating some associated genetic disorders, such as cardiovascular diseases, metabolic diseases, inflammatory diseases, and tumors by means of reversible and modulating control of gene expression epigenetics using genetic editing techniques.
基因组编辑技术自 2010 年以来通过一些技术得以发展，如成簇规律间隔短回文重复序列（CRISPR）DNA 序列/CRISPR 相关（Cas）9 系统，或通过源自一些微生物（如细菌）对质粒和病毒的宿主反应系统的遗传操作工具。CRISPR/Cas9 方法作为基因组编辑工具的引入，由于其使用的简便性和有效性以及对不同生物医学领域的高度适应性，代表了基因组编辑方法的一个重要进展。这一段将讨论所有设想的技术和新的观点，这些技术和观点可以通过使用遗传编辑技术对基因表达进行可逆和调节控制，应用于治疗一些相关的遗传疾病，如心血管疾病、代谢疾病、炎症性疾病和肿瘤。
